摘要
以采自吉林省白山市靖宇县的野生百合感病叶片为试材,采用小RNA深度测序法检测到1株百合斑驳病毒(Lily mottle virus,LMoV),采用分段克隆方法,对LMoV靖宇分离物(LMoV-JY)的全基因组进行测定并分析,以期对吉林省百合病毒病的检测和防控提供参考依据。结果表明:LMoV-JY基因组大小为9648个核苷酸(nt)序列(GenBank登录号MT795719),该核苷酸序列在第154~9444位存在1个大的开放阅读框(ORF),编码1个多聚蛋白(分子量351.46kD)。LMoV-JY与GenBank中登录的其他LMoV分离物的全基因组核苷酸序列比较,其一致性为82.82%~97.85%,在氨基酸水平上的一致性为92.96%~98.64%,其中与百合斑驳病毒大连分离物LMoV-DL(HM222521)的一致性在该2种水平上均为最高。对比LMoV-JY外壳蛋白与其他54个分离物的氨基酸序列,发现可将LMoV分离物分为2个类群。
The infected leaves of wild lily were taken from Jingyu county,Baishan city,Jilin Province.Lily mottle virus(LMoV)was detected by small RNA deep sequencing and the whole genome of LMoV Baishan-Jingyu isolate(LMOV-JY)was determined and analyzed by segmentation cloning method,in order to provide reference for detection,prevention and control of lily virus disease in Jilin Province.The results showed that the genome of LMoV-JY consisted of 9648 nucleotides(nt)(Genbank Accession No.MT795719).A large open reading frame(ORF)located at nt positions from 154 to 9444 which encoding a polypeptide of 351.46 kD.By compairing with other isolates of LMoV,the nucleotide identity was from 82.82%to 97.85%,and the amino acid identity was from 92.96%to 98.64%,and it shared the highest identity with the Dalian isolate LMoV-DL(HM222521).Amino acid sequences comparison of the CP gene of LMoV-JY with other 54 isolates in GenBank showed that all of the isolates could be divided into two subgroups.
作者
张春雨
李小宇
王晨
黄瑞贤
黄淑敏
王永志
ZHANG Chunyu;LI Xiaoyu;WANG Chen;HUANG Ruixian;HUANG Shumin;WANG Yongzhi(Institute of Plant Protection,Jilin Academy of Agricultural Sciences(Northeast Agricultural Research Center of China),Gongzhuling,Jilin 136100;Jingyu Agricultural Technology Extension Center,Baishan,Jilin 135200)
出处
《北方园艺》
CAS
北大核心
2023年第24期59-64,共6页
Northern Horticulture
基金
吉林省农业科技创新工程资助项目(CXGC2021ZY022)。
关键词
百合斑驳病毒
基因组
Lily mottle virus(LMoV)
genome
