摘要
目的:探讨三维(3D)生物打印肝肿瘤模型用于中药药敏试验的可行性。方法:3D打印SMMC-7721肝肿瘤模型,用水提醇沉法提取白术和薏米醇提液,分别进行3D细胞模型药敏试验。使用钙黄绿素-AM/碘化丙啶检测细胞活死状况,并与二维(2D)培养药敏试验比较。结果:在3D肿瘤模型下,SMMC-7721细胞倾向于成球而不是2D培养中的单层细胞,二种中药均采用方差分析进行2D与3D存活率比较,P值均<0.000 1。表明3D肝肿瘤细胞培养对这二种中药的敏感性均显著低于2D培养。结论:3D肿瘤模型是一种潜在的非常有用的药物分析工具,能更真实地模拟体内肿瘤生长的微环境,有助于确认中药药敏试验的可靠性。可以在细胞实验与临床实验的联系中发挥作用,促进候选中药的药物潜力发展。
Objective:To explore the feasibility of using three-dimensional(3D)Bio-printed liver tumor model in traditional Chinese medicines(TCMs)susceptibility test.Methods:The SMMC-7721 liver tumor model was 3D printed,and the alcoholic extracts of atractylodes macrocephala and semen coicis were extracted by water extraction in combination with alcohol precipitation.Then the drug sensitivity tests of the two TCMs with 3D cell model were carried out respectively.Cadherin-AM/propidium iodide was used to measure cell viability and death,in addition,the results were compared with those of 2D culture.Results:In the 3D tumor model,SMMC-7721 cells tended to form into pellets instead of monolayer cells in 2D culture.Analysis of variance was performed to compare 2D and 3D survival rates,and the P values of the two TCMs were all less than 0.0001,indicating that the sensitivity of 3D liver tumor cell culture to the two TCMs was significantly lower than that of 2D culture.Conclusion:3D tumor model is a potentially tool for drug analysis,which can more realistically simulate the microenvironment of tumor growth in vivo and help to confirm the reliability of TCMs drug sensitivity tests.It can play a role in the connection between cell experiment and clinical experiment,and promote the drug potential development of candidate TCMs.
作者
徐怡朦
唐靓
王晗
杨亚冬
赵思雨
张文元
XU Yimeng;TANG Liang;WANG Han(Institute of Bioengineering,Hangzhou Medical College,Hangzhou City,Zhejiang Province 310013;不详)
出处
《医学理论与实践》
2021年第5期721-725,共5页
The Journal of Medical Theory and Practice
基金
浙江省中医药科技计划项目(2018ZA017,2021ZB080)
浙江省医药卫生科技计划项目(2019KY364,2020KY532,2021KY126,2021KY645)。
关键词
3D打印
3D肝癌模型
白术
薏米
药敏试验
Three-Dimensional(3D)printing
3D liver cancer model
Atractylodes macrocephala
Semen coicis
Drug sensitive test
