摘要
目的:采用水蒸气蒸馏法提取花椒精油,制备花椒精油固体制剂,并对花椒精油固体制剂的抗真菌作用进行研究,以期为中草药及食品的储藏提供安全、绿色、高效的杀菌剂。方法:采用水蒸气蒸馏法提取花椒精油,利用气相色谱-质谱法(GC-MS)对不同产地花椒(甘肃大红袍花椒、韩城大红袍花椒、汉源红花椒和茂汶大红袍花椒)精油中化学成分及其相对质量分数进行分析,选用Agilent HP-5毛细管柱,程序升温进行分离(初始温度60℃,保持2 min,以10℃·min^(-1)上升至280℃,保持5 min),扫描范围m/z 35~590。利用纳米分子筛吸附法制备花椒精油固体制剂,考察花椒精油固体制剂对黄曲霉菌及其分生孢子的抑制作用;采用超高效液相色谱-荧光检测器(UPLC-FLD)分析花椒精油固体制剂对黄曲霉毒素的抑制作用,激发波长360 nm,发射波长440 nm。结果:4个不同产地花椒的平均精油提取率5.2%;(+)-柠檬烯、芳樟醇和乙酸芳樟酯是不同产地花椒精油的主要成分。含花椒精油体积分数为0.1%时,花椒精油固体制剂对黄曲霉菌分生孢子的抑制率(16.41±8.89)%;含花椒精油体积分数为0.2%时,花椒精油固体制剂对黄曲霉菌的生长抑制率(8.11±2.70)%;当花椒精油固体制剂含花椒精油体积分数为0.5%时,对黄曲霉菌的生长抑制率(21.62±5.41)%,对黄曲霉菌分生孢子的抑制率(45.43±5.67)%,对黄曲霉毒素的抑制作用可达(90.47±12.77)%。结论:不同产地花椒精油的化学成分存在一定差异。花椒精油对黄曲霉菌分生孢子的形成及黄曲霉毒素B1的产生均有抑制作用,可将花椒精油开发成抑菌制剂,运用于中药材、食品的存储。
Objective: To extract essential oil of Zanthoxyli Pericarpium,to prepare Zanthoxyli Pericarpium essential oil solid preparation and investigate its anti-fungal effect,in order to provide safe,green and efficient fungicide for the storage of Chinese herbal medicine and food. Method: The essential oil of Zanthoxyli Pericarpium was extracted by steam distillation method,gas chromatography-mass spectrometry(GC-MS)was adopted to analyze the chemical compositions and their relative contents in essential oil of Zanthoxyli Pericarpium from different producing areas,Agilent HP-5 capillary column was used for separation at programmed temperature(the initial temperature was 60 ℃,kept for 2 min,then increased to 280 ℃ by 10 ℃·min^(-1),kept for 5 min),the scanning range was m/z 35-590. Zanthoxyli Pericarpium essential oil solid preparation was prepared by nanomolecular sieve adsorption method,and its inhibitory effect on Aspergillus flavus and its conidia was investigated. Ultra-high performance liquid chromatography-fluorescence detector(UPLC-FLD)was used to analyze the inhibitory effect of Zanthoxyli Pericarpium essential oil solid preparation on aflatoxin under the conditions of excitation wavelength of 360 nm and emission wavelength of 440 nm.Result: The average extraction rate of essential oil in Zanthoxyli Pericarpium from four producing areas was5.2%.(+)-Limonene,linalool and linalyl acetate were the main components of Zanthoxyli Pericarpium essential oil from different producing areas. When the volume fraction of essential oil in the solid preparation was 0.1%,the inhibition rate of the solid preparation on the conidia of A. flavus was(16.41±8.89)%. When the volume fraction of essential oil in the solid preparation was 0.2%,the inhibition rate for the growth of A. flavus was(8.11±2.70)%. When the volume fraction of essential oil in the solid preparation was 0.5%,the inhibition rate for the growth of A. flavus was(21.62±5.41)%,the inhibition rate for A. flavus conidia was(45.43±5.67)%,and the inhibition effe
作者
周杰
刘璐
邱盛敏
杨熙贤
胡一晨
ZHOU Jie;LIU Lu;QIU Sheng-min;YANG Xi-xian;HU Yi-chen(Key Laboratory of Coarse Cereal Processing,Ministry of Agriculture and Rural Affairs,Chengdu University,Chengdu 610106,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2021年第2期153-160,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金青年科学基金项目(81603288)
四川省中医药管理局花椒专项(2018HJZX007)。
关键词
花椒
精油
化学成分
固体制剂
抗真菌
黄曲霉毒素
气相色谱-质谱法
Zanthoxyli Pericarpium
essential oil
chemical composition
solid preparation
antifungal
aflatoxin
gas chromatography-mass spectrometry(GC-MS)
