摘要
目的探讨扶土生金康复方治疗新型冠状病毒肺炎恢复期免疫抑制的作用机制。方法采用中医药网络药理学智能信息平台,检索扶土生金康复方的化学成分以及对应的靶点,通过GeneCards数据库获得免疫抑制相关靶点,将药物成分靶点与免疫抑制相关靶点对应,筛选交集靶点及相关功效成分。利用Cytoscape软件,构建“中药-成分-作用靶点-疾病”网络。通过STRING平台,进行PPI网络构建,找出核心靶点,再对交集靶点进行GO、KEGG富集分析。结果扶土生金康复方作用于免疫抑制的活性成分主要为槲皮素、木犀草素、山柰酚、柚皮素等,关键靶点为mTOR、TLR2、TNF、AKT1、MAPK3等,GO与KEGG富集分析提示通过T细胞活化、氧化应激等参与体内生物过程以及调控PI3K-Akt信号通路、EGFR酪氨酸激酶抑制剂耐药通路等方式调节免疫功能。结论阐述了扶土生金康复方治疗新型冠状病毒肺炎恢复期免疫抑制的作用机制,为未来研究提供了理论基础和研究方向。
OBJECTIVE To explore the mechanism of Futu Shengjin rehabilitation formula in the treatment of immunosuppression during COVID-19 convalescence.METHODS Using the TCM network pharmacology intelligent information platform,the chemical components and corresponding targets of Futu Shengjin rehabilitative formula were retrieved.Immunosuppression-related targets were obtained through GeneCards database.The drug component targets were corresponding to immunosuppression-related targets,and the intersection targets and efficacy components were screened.Cytoscape software was used to construct a"Chinese medicine-ingredient-target-disease"network.Through the String platform,PPI network was constructed to find out the core targets,and then GO and KEGG enrichment analysis was conducted on the intersection targets.RESULTS The active ingredients of Futu Shengjin rehabilitative formula in the immunosuppression,were mainly for quercetin,luteolin,kaempferol,naringenin,key targets for propagated were mTOR,TLR2,TNF,AKT1,MAPK3.GO and KEGG enrichment analysis indicated that immune function was regulated through the T cell activation,involvement in biological processes such as oxidative stress,and regulate PI3K-Akt signal pathway,EGFR tyrosine kinase inhibitor resistant methods.CONCLUSION This study elaborates the mechanism of Futu Shengjin rehabilitation prescription in the treatment of immunosuppression during COVID-19 convalescence,providing a theoretical basis and research direction for future research.
作者
王博寒
史锁芳
张晓娜
WANG Bo-han;SHI Suo-fang;ZHANG Xiao-na(Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Nanjing,210029,China)
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2021年第1期91-95,共5页
Journal of Nanjing University of Traditional Chinese Medicine
基金
国家自然科学基金(81774267)
江苏省中医药管理局项目(BE2020617)
江苏省科技厅项目(JSZYJ202002)。
关键词
网络药理学
扶土生金康复方
新型冠状病毒肺炎恢复期
免疫抑制
作用机制
network pharmacology
Futu Shengjin rehabilitative formula
COVID-19 during convalescence
immunosuppression
mechanism of action
