摘要
目的观察湿润暴露疗法/湿润烧伤膏(MEBT/MEBO)对大鼠慢性难愈合创面组织中抵抗素样分子α(RELM-α)表达的影响。方法将135只雄性大鼠随机分为5组,每组27只54个创面模型。慢创组、MEBO组、贝复济组建立全层皮肤缺损创面后立即肌肉注射醋酸氢化可的松注射液建立慢性难愈合创面,急创组不注射醋酸氢化可的松注射液,空白组仅备皮处理而不做任何创面损伤处理。慢创组和急创组创面局部涂生理盐水,MEBO组局部涂MEBO,贝复济组局部涂贝复济,均予无菌干纱布包扎固定,每天换药2次。观察各组创面愈合情况,计算各组干预后第3,7,14天的创面愈合率,采用Western blot和qRT-PCR技术检测第3,7,14天创面组织中RELM-α表达情况。结果干预后第7天,急创组、MEBO组、贝复济组局部见明显鲜红肉芽组织爬行,而慢创组肉芽灰暗;干预后第14天,急创组、MEBO组和贝复济组创面均可见表皮及毛发覆盖,局部干燥;慢创组局部肉芽灰暗、水肿,局部可见渗出液。干预后第7天和第14天,急创组、MEBO组和贝复济组创面愈合率均明显高于慢创组(P均<0.05)。干预后第3天开始,除空白组外,其余组创面组织中RELM-α蛋白和RELM-αmRNA表达水平均逐渐升高(P均<0.05),但MEBO组、贝复济组RELM-α蛋白和RELM-αmRNA表达水平均明显低于同期慢创组(P均<0.05),MEBO组与贝复济组比较差异均无统计学意义(P均>0.05)。结论MEBT/MEBO促进慢性创面愈合机制可能与调控RELM-α动态表达从而调节成纤维细胞沉积和新生血管再生有关。
Objective It is to observe the effect of MEBT/MEBO on the expression of resistin-like moleculeα(RELM-α)in chronic refractory wounds in rats.Methods 135 male rats were randomly divided into 5 groups,with 27 models of 54 wounds in each group.The chronic wound group,MEBO group,and Beifuji group were injected with hydrocortisone acetate injection immediately after the full-thickness skin defect wound was established to establish chronic refractory wounds.The acute wound group was not injected with hydrocortisone acetate injection,and the blank group only prepared skin without any wound treatment.The wounds in the chronic wound group and acute wound groups were locally coated with saline,MEBO group was locally coated with MEBO,and Beifuji group was locally coated with Beifuji.All the groups were bandaged and fixed with sterile dry gauze,and the dressing was changed twice a day.The wound healing of each group was observed,and the wound healing rate of each group was calculated on the 3rd,7th,and 14th day after intervention.Western blot and qRT-PCR were used to detect the expression of RELM-αin the wound on the 3rd,7th and 14th day.Results On the 7th day of the intervention,local bright red granulation tissue crawling was found in the acute wound group,MEBO group,and Beifuji group,while the granulation in the chronic wound group was gray;on the 14th day of the intervention,the wounds were covered with epidermis and hair in the acute wound group,MEBO group and Beifuji group,and the local area was dry;the local granulation in the chronic wound group was dull,edema,and local exudate was seen.On the 7th and 14th day of the intervention,the wound healing rate of the acute wound group,MEBO group and Beifuji group was significantly higher than that of the chronic wound group(all P<0.05).From the 3rd day after intervention,the expression levels of RELM-αprotein and RELM-αmRNA in wound tissues of all the groups except for the blank group were gradually increased(P<0.05),but the expression levels of RELM-αprotein and RELM-�
作者
黄许森
兰海生
李明尚
黄海舸
岑小宁
吕建生
唐乾利
HUANG Xusen;LAN Haisheng;LI Mingshang;HUANG Haige;CEN Xiaoning;LYU Jiansheng;TANG Qianli(Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000,Guangxi, China;The Clinical Medical College of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)
出处
《现代中西医结合杂志》
CAS
2021年第1期7-11,73,共6页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
国家自然科学基金项目(81860852)
广西自然科学基金项目(2017GXNSFAA198321)
广西特聘专家专项经费资助项目(桂人才通字【2019】13号)
广西医学高层次领军人才培养“139”计划资助项目(桂卫科教发[2018]22号)。
