摘要
目的:观察蛋白激酶C转导途径对3T3-L1脂肪细胞抵抗素表达的影响。方法:3T3-L1脂肪细胞诱导分化为成熟的脂肪细胞后,分别于培养瓶中加入浓度为50 nmol/L的12-肉豆蔻酰-13-乙酸佛波酯(PMA)和浓度为5μmol/L马来酰亚胺甲磺酸盐(Ro-31-8220),培养24 h,用RT-PCR的方法检测3T3-L1脂肪细胞抵抗素mRNA的表达,用W estern b lotting检测3T3-L1脂肪细胞抵抗素表达。结果:PMA组可以明显提高3T3-L1脂肪细胞抵抗素基因及蛋白的表达,明显高于对照组,两者的差异显著(P<0.01);Ro-31-8220组其表达低于对照组,两者的差异显著(P<0.01)。结论:蛋白激酶C转导途径可以调控3T3-L1脂肪细胞抵抗素的表达。
AIM： To investigate the effect of protein kinase C on resistin expression in 3T3 - L1 adipocytes. METHODS： The differentiated 3T3 - L1 adipocytes were incubated with 50 nmol/L phorbol 12 - myristate 13 - acetate （PMA） or 5 μmol/L Ro - 31 - 8220 for 24 h. Expression of resistin mRNA was detected by RT - PCR and expression of resistin protein was detected by Western blotting. RESULTS： Compared with control, PMA increased the expression of resistin mRNA and protein in 3T3 -L1 adipocytes significantly （P 〈 0. 01 ） , while Ro -31 -8220 decreased the expression of resistin mRNA and protein in 3T3 - L1 adipocytes obviously （ P 〈 0.01 ）. CONCLUSION ： Protein kinase C signal pathway may regulate resistin expression in 3T3 - L1 adipocytes.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第8期1640-1643,共4页
Chinese Journal of Pathophysiology
