摘要
目的了解昆明地区急性下呼吸道感染(acute lower respiratory tract infection,ALRTI)住院患儿人鼻病毒(human rhinovirus,HRV)感染的分子流行病学特征。方法选取2019年1月至2019年12月在昆明市儿童医院住院的ALRTI患儿共2 260例作为研究对象,利用逆转录聚合酶链式反应(RT-PCR)对其鼻咽抽吸物或痰液进行HRV检测,随机选取40份HRV检测阳性标本扩增VP4/VP2基因片段,并对扩增产物进行测序分型。结果 ALRTI患儿HRV检出率为6.50%(147/2 260)。不同年龄段ALRTI患儿HRV检出率不存在差异(χ^(2)=3.240,P=0.356)。不同季节ALRTI患儿HRV检出率存在差异,夏季最高,其次为秋季(χ^(2)=37.674,P <0.05)。随机检测的40份阳性标本中,23份为HRV-A型,5份为HRV-B型,12份为HRV-C型。HRV检测阳性与HRV检测阴性患儿的临床表现在发热、咳嗽方面,差异无统计学意义(P> 0.05),但在喘息方面,差异有统计学意义(P <0.05)。结论 HRV是昆明地区急性下呼吸道感染的病原体之一,流行季节在夏秋季,以HRVA型为主,感染HRV患儿更容易出现喘息。
Objective To investigate the molecular epidemiological characteristics of human rhinovirus(HRV)infection in hospitalized children with acute lower respiratory tract infection(ALRTI)in Kunming.Methods A total of 2260 children with ALRTI who were hospitalized in Kunming children's Hospital from January 2019 to December 2019 were selected.HRV was detected by reverse transcription polymerase chain reaction(RTPCR)in nasopharyngeal aspirates or sputum.40 HRV positive samples were randomly selected to amplify VP4/VP2 gene fragment,and the amplified products were sequenced and their types were identified.Results The positive rate of HRV was 6.50%(147/2260).There was no significant difference in HRV positive rate among different age groups(χ^(2)=3.240,P=0.356).The positive rate of HRV in children with ALRTI was significantly different in different seasons,the peak is in summer,followed by autumn(χ^(2)=37.674,P<0.05).Among the 40 positive samples,23 were HRV-A,5 were HRV-B and 12 were HRV-C.There was no significant difference in fever and cough between HRV positive and HRV negative children(P>0.05),but there was significant difference in wheezing(P<0.05).Conclusion HRV is one of the pathogens of acute lower respiratory tract infection in Kunming area.The epidemic season is summer and autumn and HRV-A is the main type.Children infected with HRV are more likely to have wheezing.
作者
谭力
李明
孙宇星
张桢
甘政艳
TAN Li;LI Ming;SUN Yu-xing;ZHANG Zhen;GAN Zheng-yan(Dept.of Respiratory Medicine,Kunming Children’s Hospital;Dept.of Respiratory Medicine,Children’s Hospital Affiliated to Kunming Medical University,Kunming Yunnan 650228,China)
出处
《昆明医科大学学报》
CAS
2020年第12期119-122,共4页
Journal of Kunming Medical University
基金
昆明市科技计划基金资助项目(2017-1-S-16595)
昆明市卫生科技基金资助项目(2020-06-01-114)。
关键词
鼻病毒
呼吸道感染
儿童
流行病学
Rhinovirus
Respiratory tract infection
Children
Epidemiology
