摘要
目的了解2018年上海地区呼吸道感染流感样病例中人鼻病毒(human rhinovirus,HRV)流行状况和基因分型特征。方法对2018年1—12月上海地区8家国家级流感监测哨点医院采集的960件流感样病例鼻咽拭子标本,采用逆转录实时荧光定量PCR方法进行HRV核酸筛查。所有HRV核酸阳性标本通过RT-PCR扩增其VP4/VP2基因片段,并测序,对获得的基因序列进行特征分析。结果960件流感样病例鼻咽拭子标本中,HRV核酸阳性检出62件,检出率为6.46%(62/960),其中对56条VP2/VP4基因序列进行分型,51条为HRV-A型,1条为HRV-B型,4条为HRV-C型。在HRV感染阳性病例中10岁以下儿童为14.61%(13/89),青壮年和老年人中所占比例在2.70%~8.97%之间。1—3月未检出HRV,9—11月检出率较高,为62.90%。结论2018年上海地区流感样病例中HRV感染主要在秋季,10岁以下儿童高发,HRV-A型是主要型别。
Objective To investigate the prevalence characters and genotypes of human rhinovirus(HRV)among influenza-like illness(ILI)patients in Shanghai in 2018.Methods The pharyngeal swab samples of 960 influenza-like cases in eight national sentinel hospitals were collected from January to December 2018.The HRV RNA was screened by realtime quantitative RT-PCR.The nucleic acid positive samples were used in amplification and sequencing of the VP4/VP2 gene segments.Character analysis and genotyping were performed based on the obtained sequences.Results Sixty-two samples of the 960 ILI case were positive for HRV RNA and the positive rate was 6.46%(62/960).Among the 56 sequences of VP4/VP2 genes,51 were HRV-A,1 was HRV-B,and 4 were HRV-C.For the HRV infected cases,patients aged 0-10 years accounted for 14.61%(13/89).Young adults and the elderly people accounted between 2.70%to 8.97%.No HRV infection was found from January to March,and the positive rate of HRV from September to November was 62.90%.Conclusions The HRV was mainly found in autumn among ILI patients in Shanghai in 2018.High prevalence of HRV in children less than 10 years old was observed.HRV-A is the dominant genotype.
作者
崔晓青
滕峥
周艳秋
赵雪
张曦
Cui Xiaoqing;Teng Zheng;Zhou Yanqiu;Zhao Xue;Zhang Xi(Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China)
出处
《国际病毒学杂志》
2020年第4期279-282,共4页
International Journal of Virology
基金
十三五国家传染病防治科技重大专项上海市传染病病原谱及流行规律研究(2017ZX10103009-003)
上海市卫生和计划生育委员会青年科研课题(20154Y0165)。
关键词
人鼻病毒
分子流行病学
基因特征
Human rhinovirus
Molecular epidemiology
Genetic characteristic
