摘要
目的了解SARS-CoV-2流行后,疫情常态化防控措施对本地区住院儿童其他呼吸道感染病原体的影响。方法纳入上海市儿童医院2019年8月1日—2022年2月28日,临床诊断为急性呼吸道感染(acute respiratory tract infections,ARTIs)的住院患儿咽拭子标本6020例。使用多重PCR与毛细电泳法对11种与ARTIs相关的病毒与非典型病原体进行检测。以2019年8—12月数据作为疫情前,以2020年8—12月和2021年8—12月数据分别作为疫情常态化后进行比较。以2020年3月—2022年2月的数据作为COVID-19疫情常态化后不同季节儿童呼吸道病原体进行比较,并对其流行病学进行分析。结果6020例ARTIs住院患儿标本中,病原体阳性检出率为62.34%(3753/6020)。其中人鼻病毒(human rhinovirus,HRV)检出率最高,为22.76%(1442/6020),其次为人呼吸道合胞病毒(human respiratory syncytial virus,HRSV)为16.05%(966/6020)。2019年、2020年和2021年的8—12月单个呼吸道病原体检出率依次为87.94%(569/647)、66.21%(480/725)、60.33%(1075/1782),混合感染率依次为25.66%(166/647)、9.93%(72/725)、8.87%(158/1782),呈逐年下降趋势(χ^(2)=165.19和127.79,P<0.01)。相较疫情前,人偏肺病毒(human metapneumovirus,HMPV)、HRV和人副流感病毒(human parainfluenza virus,HPIV)2020年检出率最高,分别为4.97%(36/725)、34.21%(248/725)和14.48%(105/725)(χ^(2)=26.16、42.04和60.52,P<0.01);HRSV 2021年检出率最高,为21.72%(387/1782)(χ^(2)=44.26,P<0.01)。2020年3月—2022年2月疫情常态化后,2020年病原体检出率为64.49%(1340/2078),显著高于2021年的57.48%(1771/3081)(χ^(2)=25.43,P<0.01)。其中2020年春季仅检出肺炎支原体和人冠状病毒两种呼吸道病原体,HRV、人腺病毒和HPIV从夏季开始检出,乙型流感病毒从2021年春季开始检出,甲型流感病毒仅在2020年检出1例,其余呼吸道病原体从2020年秋季开始检出。混合感染病原体以HRV+HRSV为主。结论SARS-CoV-2流行后采取的一系列防控�
Objective To investigate the impact of SARS-CoV-2 pandemic on the prevalence of viral pathogens in hospitalized children with acute respiratory tract infections(ARTIs)in Shanghai.Methods A total of 6020 throat swab specimens were collected from hospitalized children with ARTIs in Shanghai Children′s Hospital from August 1,2019 to February 28,2022.Eleven common respiratory pathogens were detected using multiplex PCR and capillary electrophoresis.Pre-epidemic data referred to the data from August to December,2019,and the data from August to December,2020 and August to December,2021 were used as the post-epidemic data for comparison.Based on the data from March 2020 to February 2022(the epidemic period),the epidemiology of respiratory pathogens in children with ARTIs in different seasons were compared(spring:March to May,summer:June to August,autumn:September to November,winter:December to February of the next year).Results Of the 6020 specimens obtained from the patients,3753(62.34%)were positive for at least one pathogen.Human rhinovirus(HRV)was the most commonly detected pathogen(22.76%,1442/6020),followed by human respiratory syncytial virus(HRSV)(16.05%,966/6020).From August to December,the detection rate of single respiratory pathogen was 87.94%(569/647)in 2019,66.21%(480/725)in 2020 and 60.33%(1075/1782)in 2021,and the co-infection rate was 25.66%(166/647)in 2019,9.93%(72/725)in 2020 and 8.87%(158/1782)in 2021,showing a decreasing trend(χ^(2)=165.19 and 127.79,P<0.01).Compared with the pre-epidemic period,human metapneumovirus(HMPV),HRV and human parainfluenza virus(HPIV)were the most prevalent pathogens in 2020[4.97%(36/725),34.21%(248/725)and 14.48%(105/725);χ^(2)=26.16,42.04 and 60.52;P<0.01]and HRSV was the predominant pathogen in 2021[21.27%(387/1782),χ^(2)=44.26,P<0.01].During the epidemic period,the detection rate of pathogens was 64.49%(1340/2078)in 2020,which was significantly higher than that in 2021[57.48%(1771/3081),χ^(2)=25.43,P<0.01].Only two respiratory pathogens,Mycoplasma pneumonia and
作者
孟磊俊
王洁
蔡沁
张泓
Meng Leijun;Wang Jie;Cai Qin;Zhang Hong(Department of Clinical Laboratory,Shanghai Children′s Hospital,Children′s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200040,China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2023年第3期222-229,共8页
Chinese Journal of Microbiology and Immunology
基金
上海市临床重点专科建设项目(shslczdzk06902)。
