摘要
探究肉苁蓉水提物体外抗氧化能力及对小鼠肠道菌群紊乱的作用。以还原力、DPPH自由基、ABTS+自由基、O2-自由基清除率考察肉苁蓉水提物体外抗氧化能力。以肠道菌群,脏器指数,结肠绒毛形态结构,探究肉苁蓉水提物对肠道菌群紊乱作用。结果表明:肉苁蓉水提物抗氧化能力与其浓度呈正相关,DPPH自由基、ABTS+自由基、O2-自由基的IC50值依次为:0.225、1.819、12.53 mg/mL。肉苁蓉水提物能够缓解抗生素导致的小鼠体质量下降及对胸腺的生长抑制,显著提高肠道菌群紊乱小鼠粪便中双岐杆菌、乳酸菌含量及B/E值,对肠球菌及大肠杆菌的生长具有一定抑制作用,改善肠道菌群紊乱小鼠诱发的肠绒毛变短、隐窝深度增加的现象。肉苁蓉水提物具有良好的体外抗氧化作用,能够调节肠道菌群紊乱的作用。
To explore the antioxidant capacity of Cistanche deserticola water extract in vitro and its effect on intestinal flora disorder in mice.The antioxidant capacity of Cistanche deserticola aqueous extract in vitro was determined by the reduction of force,DPPH·,ABTS+·,O2-scavenging rate.The intestinal flora,organ index,and morphology of colonic villi were used to investigate the effect of Cistanche deserticola extract on intestinal flora.he antioxidant capacity of Cistanche deserticolata water extract was positively correlated with its concentration.The IC50 values of DPPH·,ABTS+·,and O2-were 0.225,1.819,12.53 mg/mL.Cistanche water extract could alleviate the decrease in body weight of mice caused by antibiotics and inhibit the growth of thymus,and significantly increase the content of Bifidobacterium,lactic acid bacteria and B/E in feces of mice with intestinal flora disturbance.It had a certain inhibitory effect and improves the phenomenon of intestinal villi shortening and crypt depth increase induced by mice with intestinal flora disturbance.Cistanche deserticolata water extract had a good antioxidant effect in vitro,it could regulate the intestinal flora disturbance.
作者
何梦梦
游林
包晓玮
魏晨业
肖子轩
李泽霖
曾兰君
王慧君
赵玉婷
HE Meng-meng;YOU Lin;BAO Xiao-wei;WEI Chen-ye;XIAO Zi-xuan;LI Ze-lin;ZENG Lan-jun;WANG Hui-jun;ZHAO Yu-ting(College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China;Hetian Dichen Medical Biotechnology Co.,Ltd.,Hetian 848000,Xinjiang,China)
出处
《食品研究与开发》
CAS
北大核心
2020年第23期44-50,共7页
Food Research and Development
基金
新疆维吾尔自治区边远贫困县市科技人员专项支持计划。
关键词
肉苁蓉
水提物
体外抗氧化
肠道菌群
结肠绒毛形态结构
Cistanche
water extract
antioxidant in vitro
intestinal flora
morphology of colonic villi
