摘要
为探究桑(Morus alba)叶青贮中微生物的菌群结构与变化及直接青贮效果不佳的原因,以可溶性糖与乳酸菌为改良方向,研究了桑叶在青贮中微生物动态变化特点,并对青贮品质进行弗氏评价。试验设置桑叶玉米粉组(M)、桑叶乳酸菌组(J)和桑叶组(对照Y)3组,呼吸膜袋式青贮,分别在青贮第1、3、5、7、10、14、20、28、38、56天开袋取样,采用微生物分离培养和二代测序法分析不同处理组的微生物变化并检测青贮饲料的品质。结果表明:乳酸菌或玉米粉的添加使桑叶中粗蛋白含量显著降低(P<0.05),可溶性糖含量显著增加(P<0.05),但对主要微生物数量的影响较小。添加剂缩短了桑叶青贮中的好氧呼吸期和乳酸发酵期,提高了厚壁菌门(Firmicutes)的丰度与乳酸菌数量,青贮品质优劣顺序为M>J>Y。结果表明,乳酸菌与玉米粉均对桑叶青贮品质有改善作用,玉米粉最佳。
This study aimed to explore the reasons for changes in the microbial flora composition and in mulberry leaf silage,specifically,the reasons for the poor direct silage effect.We focused on the improvement of soluble sugar content and lactic acid bacterial counts.The dynamic characteristics and quality of mulberry leaves in silage were studied.Three experimental groups were evaluated:Mulberry leaf cornflour group(M),mulberry leaf lactic acid bacteria group(J),and mulberry leaf group(control Y).We used respiratory membrane bag silage,and sampling was performed on days 1,3,5,7,10,14,20,28,38,and 56 of ensilage.Microbial isolation culture and second-generation sequencing methods were used to analyze the microbial changes across treatment groups and to detect silage quality.The addition of lactic acid bacteria and cornflour significantly reduced the crude protein content in mulberry leaves(P<0.05)and increased(P<0.05)the soluble sugar content.However,this had a small impact on the number of major microorganisms.Additives shortened the aerobic respiration and lactic acid fermentation periods in mulberry leaf silage and increased the abundance of Firmicutes and lactic acid bacteria.The order of silage quality was M>J>Y.Lactic acid bacteria and cornflour both improved mulberry leaf silage quality,with the latter having a more pronounced effect.
作者
徐泽平
肖润
沈诗桀
沈以红
黄先智
向钊
XU Zeping;XIAO Run;SHEN Shijie;SHEN Yihong;HUANG Xianzhi;XIANG Zhao(College of Animal Science,Southwest University,Chongqing 402460,China;State Key Laboratory of Silkworm Genomic Biology,Southwest University,Chongqing 400715,China;Key Laboratory of Sericulture Functional Genomics and Biotechnology,Ministry of Agriculture,Chongqing 400715,China)
出处
《草业科学》
CAS
CSCD
2020年第9期1912-1925,共14页
Pratacultural Science
基金
农业部现代农业产业技术体系建设专项(CARS-18)
公益性行业(农业)科研专项(201403049)
重庆市科委社会民生重点项目(CSTS2017shms-zdyfx0054)。
关键词
桑叶
青贮
微生物
二代测序
mulberry leaf
silage
microorganism
next-generation sequencing
