摘要
高通量技术是以微型生物反应器为操作平台,实现对生物细胞的分离培养和分子测序。通过着重分析几种实用型高通量技术在微生物分离培养及分子测序中的应用进展,发现微包埋技术在高通量试验中的研究及应用较为深入;而对不同环境菌样的分离培养中,海洋微生物方面的应用最为突出。另外,通过对比高通量分子测序技术可以发现,Illumina测序法的平均测序速度最快;单分子测序法所得数据的精确度最高,测序读长最长,周期相对最短。最后,对高通量技术在微生物学应用中的一些不足进行总结,并提出假设性解决方法。
High-throughput technology is based on microbioreactor to realize the separation,culture and molecular sequencing of biological cells.According to the analysis of the application of several practical high-throughput techniques in microbial isolation and culture,it was found that the micro encapsulation technology was deeply studied and applied in high-throughput experiments.The application of marine microorganism is the most prominent in the isolation and culture of bacteria samples from different environments.In addition,by comparing the high-throughput molecular sequencing technique,we can find that the Illumina sequencing method has the fastest average sequencing speed,and the single molecule sequencing method has the highest accuracy,the longest reading length and the shortest cycle.Finally,we summarized the shortcomings of high-throughput technology in microbiology,and proposed hypothetical solutions.
作者
刘莹
韩锰
王文磊
马成玉
张沛
LIU Ying;HAN Meng;WANG Wen-lei(Henan Center for Supervision&Inspection of Grain,Oil and Feed Product Quality,Zhengzhou,Henan 450001;National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan,Hubei 430070;Nanjing Argricultural University,Nanjing,Jiangsu 210095)
出处
《安徽农业科学》
CAS
2020年第15期16-19,共4页
Journal of Anhui Agricultural Sciences
基金
国家自然基金青年项目(31701121)
河南省科技公关项目(182102310667)
河南省高等学校重点科研项目计划(18A180022)。
关键词
微生物
高通量技术
分离培养
分子测序
Microorganism
High-throughput technology
Isolation and culture
Molecular sequencing
