摘要
马铃薯土传病害日益严重,对病原菌进行快速检测是病害早期诊断、预测监控、综合防治的重要基础。本文综合比较了普通PCR、巢式PCR、实时荧光定量PCR、多重PCR和环介导等温扩增技术(LAMP)在灵敏度和特异性等方面的特点,以及在检测马铃薯土传病害时的优缺点;总结了各病害检测报道的引物信息和检测体系的优势。同时,对各技术的应用前景进行展望,旨在对马铃薯土传病害的快速检测方法及其发展趋势有一个全面的了解。
Potato soil-borne diseases are becoming increasingly serious.Rapid detection on pathogenic bacteria is an important basis for early diagnosis,predictive monitoring,and comprehensive control of the diseases.This paper comprehensively compared the characteristics of conventional PCR,nested PCR,realtime quantitative PCR,multiplex PCR and LAMP technology in sensitivity and specificity,and evaluated their merits and demerits in detecting potato soil-borne diseases.The paper also summarized the primer information of various diseases detection reports and advantages of this detection system.At the same time,the paper prospected the applying future of each technology,aiming at obtaining an overall understanding about the rapid detection methods and their development tendency on potato soil-borne diseases.
作者
李磊
赵昱榕
谢学文
石延霞
柴阿丽
李宝聚
LI Lei;ZHAO Yu-rong;XIE Xue-wen;SHI Yan-xia;CHAI A-li;LI Bao-ju(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《中国蔬菜》
北大核心
2020年第10期27-34,共8页
China Vegetables
基金
国家重点研发计划项目(2018YFD020080703)
中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)
农业农村部园艺作物生物学与种质创制重点实验室项目。
