摘要
为提高柑橘黄龙病PCR检测方法的灵敏度,便于柑橘黄龙病的早期检测,对影响PCR检测的引物设计的特异性、退火温度、不同植物材料DNA的提取等因素进行了研究。经多次平行PCR比较实验,筛选出对亚洲韧皮杆菌有稳定的PCR扩增效率的563bp OL1/OL2引物。同时,研究结果还表明,以柑橘枝梢韧皮部为材料提取DNA的PCR检出率比叶片高。
In order to improve the sensitivity of PCR detection method for citrus Huanglongbing(HLB),thereby being convenient for detecting HLB at early stage,the factors which will affect PCR detection were studied,such as specificity of primer design,annealing temperature,extraction of DNA from different plant materials,etc..Through multiple PCR comparison experiments,a 563 bp long of primer OL1/OL2,which had stable PCR amplification efficiency to Candidatus liberibacter asiaticus,was screened.Meanwhile,the results also indicated that the PCR detecting rate for DNA extracted from phloem of citrus shoots was higher than that from citrus leaves.
出处
《湖南农业科学》
2013年第8期13-15,19,共4页
Hunan Agricultural Sciences
关键词
柑橘黄龙病
PCR
引物
退火温度
citrus Huanglongbing(HLB)
PCR
primer
annealing temperature
