摘要
目的观察联用大黄、黄芩有效部位对牙周炎SD大鼠炎性因子前列腺素E2(PGE2)、C反应蛋白(CRP)的抑制作用及骨组织再生的促进效应。方法采用结扎丝和牙周细菌联用建立SD大鼠实验性牙周炎动物模型,将100只大鼠随机分为假手术组(Sham组)、牙周炎组(P组)、大黄有效部位组(DH)、黄芩有效部位组(HQ)和大黄、黄芩配伍组(DH+HQ),分别局部注射药物进行治疗。给药4周后观察组织免疫病理,通过ELISA法检测炎症因子PGE2和CRP含量,并比较骨再生情况和骨形态学参数的变化。结果SD大鼠建模后炎症明显,有大量破骨细胞形成和明显牙槽骨吸收,龈沟液、唾液及血液中PGE2水平和血液CRP水平明显升高(P<0.05);局部DH+HQ配伍用药可增加成骨细胞形成,明显降低PGE2和CRP水平,升高骨组织形态计量学各参数,使得局部牙槽骨再生(P<0.05)。结论DH+HQ活性部位联用可以明显降低牙周炎SD大鼠炎症因子含量,有效改善骨组织形态学参数,增加成骨效果,且疗效优于单独用大黄或黄芩活性部位组。
Objective To determine the effects of Rheum officinale in combination with Scutellaria baicalensis extracts on the inhibition of prostaglandin E2(PGE2),C-reactive protein(CRP)and the promotion of bone tissue regeneration in SD rats with periodontitis.Methods Periodontitis models in SD rats were prepared by using wire ligatures and P.g W83 bacterial.A hundred rats were randomly divided into 5 groups:sham-operation group(Sham),periodontitis(P),Rheum officinale extracts(DH),Scutellaria baicalensis extracts(HQ),Rheum officinale in combination with Scutellaria baicalensis extracts(DH+HQ).The images of immunopathology,the contents of PGE2 and CRP and the regeneration of alveolar bone were measured after 4 weeks using different drugs.Results After building periodontitis models in SD rats,the inflammation symptoms and bone resorption of periodontium were obvious,and the contents of PGE2 and CRP were also higher than Sham group(P<0.05).The osteoblast formation was increased,while the levels of PGE2 and CRP were significantly reduced.The bone morphometry parameters and local alveolar bone regeneration also increased in DH+HQ combination group compared with other groups(P<0.05).Conclusion Combined application of Rheum officinale and Scutellaria baicalensis extracts can remarkably reduce the contents of PGE2 and CRP,improve the parameters of bone histomorphometry and increase bone regeration,which is better than single application of Rheum officinale or Scutellaria baicalensis extracts.
作者
谷冬华
郭宁
赵晓丹
刘瑾
李昂
苟建重
GU Donghua;GUO Ning;ZHAO Xiaodan;LIU Jin;LI Ang;GOU Jianzhong(Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,Xi’an,Department of Periodontology,College of Stomatology,Xi’an Jiaotong University,Xi’an 710004,China)
出处
《口腔医学》
CAS
2020年第8期692-697,共6页
Stomatology
基金
国家自然科学基金青年基金(81704128)
西安交通大学口腔医院院青年科研基金(201601)
西安交通大学基本业务费自由探索-学生类(xzy022019070)。
关键词
牙周炎
大黄
黄芩
前列腺素E2
C反应蛋白
骨组织形态计量学参数
显微CT
periodontitis
Rheum officinale
Scutellaria baicalensis
prostaglandin E2
C-reactive protein
the parameters of bone histomorphometry
Micro CT
