摘要
目的:体外探讨表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)对人牙髓干细胞(dental pulp stem cells,DPSCs)增殖和迁移的影响。方法:从人牙髓组织分离培养DPSCs,并通过形态学、三向分化实验及流式细胞术进行鉴定。通过CCK-8、Transwell实验和划痕实验检测EGCG对DPSCs体外增殖和迁移的影响。结果:分离培养的DPSCs呈长梭形,贴壁生长,具备三向分化潜能,且CD73、CD90和CD105阳性表达,CD34阴性表达,表明分离获得的DPSCs为间充质干细胞。2.5~20μmol/L的EGCG具有促进DPSCs增殖的作用,且在5μmol/L时显著增强细胞迁移。结论:低浓度EGCG可促进DPSCs的增殖和迁移能力,为EGCG在基于细胞归巢的牙髓-牙本质复合体再生领域的应用提供参考。
Objective:To investigate the effects of epigallocatechin gallate(EGCG)on the proliferation and migration of human dental pulp stem cells(DPSCs)in vitro.Methods:DPSCs were isolated from human dental pulp tissues and characterized by morphology,three-lineage differentiation assay and flow cytometry.The effects of EGCG on the proliferation and migration of DPSCs were examined by CCK-8,Transwell and scratch assay in vitro.Results:The isolated DPSCs were long spindle-shaped,adherent and had the potential of three-lineage differentiation.CD73,CD90 and CD105 were expressed positively,while CD34 was expressed negatively,indicating that the isolated DPSCs were mesenchymal stem cells.2.5~20μmol/L EGCG promoted the proliferation of DPSCs,and EGCG significantly enhanced cell migration at 5μmol/L.Conclusion:Low concentration of EGCG can enhance the proliferation and migration of DPSCs,providing a reference for the application of EGCG in the field of pulp-dentin complex regeneration based on cell homing.
作者
蔡明轩
汪一帆
邹杰林
王宇洁
毛靖
石鑫
CAI Ming-xuan;WANG Yi-fan;ZOU Jie-lin;WANG Yu-jie;MAO Jing;SHI Xin(Center of Stomatology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei Wuhan 430030,China;School of Stomatology,Tongji Medical College,Huazhong University of Science and Technology,Hubei Wuhan 430030,China;Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration,Hubei Wuhan 430022,China.)
出处
《临床口腔医学杂志》
2022年第5期272-276,共5页
Journal of Clinical Stomatology
基金
口腔颌面发育与再生湖北省重点实验室青年基金(2020kqhm002)
中华口腔医学会青年基金(CSA-MW02021-03)。
