摘要
研究白光、红光、蓝光、红蓝光(1:3)、红蓝光(1:2)、红蓝光(1:1)、红蓝光(2:1)和红蓝光(3:1)8种光质在100μmol m-2 s-1的光照强度下对Chlamydomonas sp.212生长特性及油脂积累的影响.在不同光质条件下进行Chlamydomonas sp.212的培养,对其生物量、光合色素含量、碳水化合物含量、蛋白质含量、油脂积累量及脂肪酸组成等指标进行测量与综合分析.结果发现,蓝光下Chlamydomonas sp.212的生物量达到最大(0.3351 g/L),红光下其生物量最小.蓝光下Chlamydomonas sp.212的叶绿素a、叶绿素b和类胡萝卜素的含量均为最高,分别为12.26、6.34和5.94 mg/L;红光下叶绿素a、叶绿素b和类胡萝卜素的含量均较低.Chlamydomonas sp.212在红光下碳水化合物的含量最高(27.54%),蛋白质的含量最低(23.68%);蓝光下碳水化合物的含量最低(19.12%),蛋白质的含量为31.46%;红蓝光下碳水化合物和蛋白质的含量低于白光.Chlamydomonas sp.212在蓝光下油脂含量为29.86%,产量为100.06mg/L;红光下油脂含量为27.52%,油脂产量最低;而红蓝光下油脂的积累量明显升高,红蓝光(1:2)下油脂含量最高35.25%,红蓝光(1:3)下油脂产量最高(109.23 mg/L).不同光质对Chlamydomonas sp.212脂肪酸组成的影响不显著,C16和C18脂肪酸的相对含量达到93%以上,饱和脂肪酸约占47%,不饱和脂肪酸约占53%.本研究表明蓝光有利于Chlamydomonas sp.212的生长,红光不利于其生长;蓝光有利于油脂的积累,红蓝光下油脂的积累显著升高,且蓝光占比越高,越有利于油脂的积累,红蓝光(1:3)下油脂的积累量最高;结果可为不同光质在微藻生产生物柴油中的应用提供理论依据.(图6表1参32)
The effects of white,red,and blue light,and the combination of red and blue light(1:3,1:2,1:1,2:1,or 3:1;light intensity=100μmol m-2 s-1 for all groups)on the growth characteristics and lipid accumulation of Chlamydomonas sp.212 were studied.Chlamydomonas sp.212 was cultured under the different light treatments,and the biomass,photosynthetic pigment content,carbohydrate content,protein content,lipid accumulation,and fatty acid composition were measured and comprehensively analyzed.The biomass of Chlamydomonas sp.212 reached a maximum of 0.3351 g/L under blue light and was lowest under red light.The contents of chlorophyll a,chlorophyll b,and carotenoids were highest under blue light(12.26 mg/L,6.34 mg/L,and 5.94 mg/L,respectively)and lowest under red light.Chlamydomonas sp.212 had the highest carbohydrate content(27.54%)and lowest protein content(23.68%)under red light,while it had the lowest carbohydrate content(19.12%)and highest protein content(31.46%)under blue light.The carbohydrate and protein contents in the red and blue light treatments were lower than in the white light treatment.The lipid content of Chlamydomonas sp.212 was 29.86%,and the production was 100.06 mg/L under blue light;the lipid content was 27.52%,and the production was lower under red light.Interestingly,the lipid accumulation was significantly increased under red and blue light.The lipid content was highest(35.25%)under red and blue light(1:2),and the lipid production was highest(109.23 mg/L)under red and blue light(1:3).There were no significant effects of the different light treatments on the fatty acid composition.The relative contents of C16 and C18 fatty acids were more than 93%,while the relative contents of saturated fatty acids were^47%and the relative contents of unsaturated fatty acids were^53%.Blue light was conducive to Chlamydomonas sp.212 growth,but red light was not.Blue light was also beneficial to lipid accumulation,and the lipid accumulation was significantly increased under red and blue light.Moreover,higher proportion
作者
孙建瑞
赵君峰
符丹丹
王大红
SUN Jianrui;ZHAO Junfeng;FU Dandan;WANG Dahong(College of Food and Bioengineering,Henan Engineering Research Center of Food Microbiology,Henan University of Science and Technology,Luoyang 471023,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2020年第4期1016-1022,共7页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(31401672)
河南省高等学校重点科研项目(17B180003)资助。
关键词
微藻
油脂
光质
生长特性
脂肪酸
microalgae
lipid
light quality
growth characteristics
fatty acid
