摘要
为促进马铃薯茎尖脱毒技术推广应用,以尤金、克新13、早大白、东农303、中薯5号和延薯4号6种马铃薯茎尖分生组织为试验材料,研究不同品种的马铃薯茎尖分生组织脱毒苗的成活情况。结果表明:尤金的成苗率可到达55.0%,克新13的成苗率为33.3%,早大白的成苗率仅为31.3%,东农303的成苗率为54.5%,中薯5号的成苗率为40.0%,延薯4号的成苗率可到达60.0%。表明不同品种马铃薯茎尖脱毒与成苗效果存在差异。经过酶联免疫吸附法对6个品种茎尖剥离后的组培苗进行病毒检测,结果表明,共有52份材料不含有6种主要病毒,能够应用于马铃薯的脱毒种薯生产。检测结果还表明在脱毒苗中PVS的含有率最高,应提高脱除马铃薯S病毒的技术水平。
In order to promote the popularization and application of virus-free technology of potato stem tip,the Youjin,Kexin 13,Zaodabai,Dongnong 303,Zhongshu No.5 and Yanshu No.4 six kinds of potato stem tip meristem as experimental materials,the survival of virus-free plantlets from meristem of different potato varieties were studied.The results showed that the the seedling rate of Youjin reached 55.0%,the seedling rate of Kexin13 reached 33.3%,the seedling rate of Zaodabai only was 31.3%,the seedling rate of Dongnong 303 was 54.5%,the seedling rate of Zhongshu No.5 was 40.0%,and the seedling rate of Yanshu No.4 reached 60.0%.It could be seen that there were differences in virus-free and seedling formation among different potato varieties.The virus were detected in different variety seedlings by Elisa.The results showed that 52 materials did not contain 6 main viruses and could be used in the production of virus-free potato seeds.The results also showed that the content of PVS in virus-free seedlings was the highest,so the technology level of removing potato S virus should be improved.
作者
张微
刘卫平
李志新
赵雪
付春江
ZHANG Wei;LIU Wei-ping;LI Zhi-xin;ZHAO Xue;FU Chun-jiang(Keshan Branch of Heilongjiang Academy of Agricultural Sciences,Qiqihar 161005,China)
出处
《黑龙江农业科学》
2020年第8期52-54,共3页
Heilongjiang Agricultural Sciences
基金
黑龙江省现代农业马铃薯产业技术协同创新体系项目(HNWJZTX201701)。
