摘要
以采自西藏山南地区隆子县加玉乡卡布沟的红皮马铃薯为试验材料,利用植物组织培养技术进行茎尖剥离,获得脱毒苗,并建立快繁再生体系。结果表明,红皮马铃薯组织培养不同培养阶段的适宜培养基分别为分化培养基:MS+6-BA 0.3 mg/L+NAA 0.02 mg/L;继代培养基:MS+6-BA 0.2 mg/L+NAA 0.05 mg/L;生根培养基:MS+IBA 0.2 mg/L。
Red-skinned potatoes collected from Kabugou,Jiayu Township,Longzi County of southern Tibet,were used as experimental materials to obtain virus-free seedlings by stripping the stem tips with plant tissue culture technology,and a rapid reproduction and regeneration system was established.The results showed that the suitable differentiation medium subculture medium and rooting medium was MS+6-BA 0.3 mg/L+NAA 0.02 mg/L,MS+6-BA 0.2 mg/L+NAA 0.05 mg/L,and MS+IBA 0.2 mg/L,respectively.
作者
范继红
巴桑次仁
边巴次仁
李志强
吴晓云
FAN Ji-hong;Basangciren;Bianbaciren(Beijing Vocational College of Agriculture,Beijing 102442;Tibet Shannan Vocational and Technical School,Shannan,Tibet 856000)
出处
《安徽农业科学》
CAS
2020年第3期50-53,共4页
Journal of Anhui Agricultural Sciences
基金
北京农业职业学院院级科研项目科技研发推广类(XY-YF-18-09)
西藏山南市科技局山南市2018年第一批科技三项经费项目
关键词
红皮马铃薯
脱毒
培养基
再生体系
Red-skinned potato
Virus-free
Medium
Regeneration system
