摘要
目的:为使微藻裂殖壶菌提取DHA后的废弃物藻粕能被再利用,研究其酶解产物抑制肠道炎症的功效,为替代现有抗生素提供试验依据。方法:采用胰蛋白酶水解藻粕,透析后获得肽混合物(MESH),添加到含有维生素AD的饲料里。分别以草鱼和鳗鱼为淡水养殖及海洋鱼类代表性测试动物,构建三硝基苯磺酸(TNBS)肠炎模型,并灌注等体积磷酸盐缓冲溶液(PBS)为阴性对照组,抗生素恩诺沙星为阳性对照组,分别于灌注的1,3,7 d解剖测试动物,通过疾病活动指数评分、髓过氧化物酶(MPO)活性的变化以及肠道病理组织变化等方面,对鱼肠炎程度进行综合评定。结果:经TNBS诱导,MESH组和恩诺沙星组均可预防治疗草鱼和鳗鱼的肠炎症状。其中MESH组对肠炎的预防和治疗作用比恩诺沙星组显著。结论:微藻裂殖壶菌藻粕酶解活性肽MESH以及复合饲料中的维生素AD,可有效抑制水产鱼类肠炎,替代现有抗生素,应用于治疗鱼类肠炎。
Objective:In order to enable the reuse of waste algal meal after the extraction of DHA by microalgae Schizochytrium sp.,this project conducted studies on the efficacy of its enzymatic hydrolysis products to inhibit intestinal inflammation,and laid a solid experimental foundation for the application of its replacement of existing antibiotics.Method:Trypsin was used to hydrolyze the algal meal,and the peptide mixture(MESH)was obtained after dialysis.It was added to the feed containing vitamin AD.Grass carp and eel was used for freshwater farming and marine fish as representative test animal for constructing trinitrobenzenesulfonic acid(TNBS)enteritis model.Negative control group were used of equal volume phosphate buffer solution(PBS),and positive control group were used antibiotic enrofloxacin.The test animals were dissected 1,3 days and 7 days after infusion,respectively.The disease activity index scores,changes in myeloperoxidase(MPO)activity,and changes in intestinal pathological tissues were used to comprehensively assess the degree of fish enteritis.Results:After induction by TNBS,both MESH group and enrofloxacin group could prevent and treat the enteritis symptoms of grass carp and eel.Among them,the MESH group had more significant prevention and treatment effects on enteritis than the enrofloxacin group.Conclusion:Microalgae Schizochytrium sp.meal enzymatically active peptide MESH compound feed vitamin AD can effectively inhibit aquatic fish enteritis,and can replace the existing antibiotics used in enteritis.
作者
李孟研
柴力彬
张延杰
邝丽红
李昊
Li Mengyan;Chai Libin;Zhang Yanjie;Kuang Lihong;Li Hao(College of Bioscience and Engineering,Institute of Applied Biotechnology,Fuzhou University,Fuzhou 350108;Fujian Landhowbio Biotechnology Co.,Ltd.,Fuzhou 350109)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2020年第8期56-64,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
国家“十三五”厦门市海洋经济创新发展示范项目(16CZP017SF06)。
关键词
裂殖壶菌酶解肽
肠道炎症
抗生素
Schizochytrium enzymolysis peptide
intestinal inflammation
antibiotic
