摘要
目的探索抗补体5a受体(C5aR)抗体联合大蒜素对大鼠炎性肠病(IBD)的治疗作用。方法将80只经2,4,6-三硝基苯磺酸(TNBS)诱导的IBD雄性大鼠随机分为4组:模型对照组、抗C5aR抗体治疗组、大蒜素治疗组、抗C5aR抗体联合大蒜素治疗组;另20只雄性大鼠为正常对照组。药物灌胃前和灌胃后14 d统计大鼠体质量变化和生存率,检测大鼠血清中白细胞介素-6(IL-6)和肿瘤坏死因子α(TNF-α)水平,进行结肠组织大体观察以及病理学评分。结果抗C5aR抗体联合大蒜素治疗组大鼠的体质量及生存率均高于其他治疗组,且大鼠血清中炎性因子IL-6、TNF-α的表达水平均呈明显回归趋势;结肠组织病理学评分也表明联合治疗组的效果明显优于其他治疗组(P<0.05)。结论抗C5aR抗体联合大蒜素是治疗TNBS诱导的大鼠IBD的有效方案,其疗效优于单独使用抗C5aR抗体或者大蒜素。
Objective To explore the therapeutic effect of anti-complement 5 a receptor(anti-C5aR)antibody combined with allicin on inflammatory bowel disease(IBD)in rats.Methods Eighty IBD male rats induced by 2,4,6-trinitrobenzenesulfonic acid sol(TNBS)were randomly divided into 4 groups:model control group,anti-C5aR antibody treatment group,allicin treatment group and anti-C5aR antibody combined with allicin treatment group,another 20 normal male rats were used as the normal control group.The changes in body weight and survival rate,serum interleukin 6(IL-6)and tumor necrosis factorα(TNF-α)levels,colonic tissue gross observation and pathological score were measured before and 14 d after drug intragastric administration.Results The body weight and survival rate of the rats treated with anti-C5 aR antibody combined with allicin were higher than those of other treatment groups,and the expression of inflammatory cytokines IL-6 and TNF-αin the serum of the rats treated with the combination of anti-C5aR antibody and allicin showed a significant regression trend with the time.The histopathological score of the colon also suggested that anti-C5 aR antibody combined with allicin had the best effect(P<0.05)in the treatment of IBD.Conclusion Anti-C5aR antibody combined with allicin is an effective treatment for TNBS-induced colitis,and its efficacy is better than anti-C5aR antibody or allicin alone.
作者
周亚妮
刘丹
ZHOU Yani;LIU Dan(Health Management School of Shangluo University,Shangluo 726000,Shaanxi Province China;Department of Rheumatology and Immunology,Fifth People's Hospital of Xi'an,Xi'an 710061,China)
出处
《实验动物与比较医学》
CAS
2020年第4期328-333,共6页
Laboratory Animal and Comparative Medicine
基金
陕西省高等教育教学改革研究项目(19ZY022)。
关键词
抗C5aR抗体
大蒜素
炎性肠病
2
4
6-三硝基苯磺酸
大鼠
Anti-C5aR antibody
Allicin
Inflammatory bowel disease
2
4
6-trinitrobenzenesulfonic acid sol
Rats
