摘要
该研究主要探讨对羟基苯甲酸甲酯(methylparaben,MP)对早孕小鼠子宫内膜蜕膜化的影响。从孕第1天开始,每日经口灌胃给予CD1小鼠0、10.0、62.5、250.0、1000.0 mg/kg浓度的MP后,于孕第7天处死小鼠。采用酶联免疫法(ELISA)检测血清雌孕激素水平,观察并计数着床胚胎数量,采用免疫组化法和免疫印迹法检测子宫内膜蜕膜化标志物BMP2、MMP2、MMP9、HOXA10等蛋白的表达水平。结果显示,在1000.0 mg/kg MP暴露下,小鼠孕第7天着床胚胎数量显著下降(P<0.05)。免疫组化和免疫印迹结果显示,与正常对照组相比较,1000.0 mg/kg组孕鼠蜕膜化标志物BMP2、MMP2、MMP9、HOXA10的蛋白表达水平显著降低。ELISA检测结果显示,MP暴露后孕鼠血清雌孕激素水平均明显下降(P<0.05)。该研究提示,MP孕期暴露可能影响孕鼠子宫内膜蜕膜化。
This study focused on whether MP(Methylparaben),the most widely used of PBs,had effects on endometriosis decidualization in early pregnancy,resulting in adverse pregnancy outcomes.In this study,on the first day of pregnancy,CD1 mice were given 0,10.0,62.5,250.0 and 1000.0 mg/kg of MP by continuous oral feeding.On the seventh day of pregnancy,mice were killed.Serum was collected for estrogen and progesterone level detection by ELISA.Uterine tissue was collected and embryo quantity and quality were observed.Protein expression levels of decidual markers such as BMP2,MMP2,MMP9 and HOXA10 were detected by immunohistochemistry and Western blot.Under 1000.0 mg/kg MP exposure,the number of mice embryos decreased significantly on the seventh day of pregnancy(P<0.05),which suggested that a high dose of MP had adverse effects on the process of endometrial decidualization of mice during early pregnancy.Immunohistochemistry and Western blot results showed that the high dose(1000.0 mg/kg)compared with 0 mg/kg group,protein expression of decidual markers BMP2,MMP2,MMP9,and HOXA10 significantly reduced.Serum estrogen and progesterone levels also decreased obviously by ELISA(P<0.05).Exposure to high dose(1000.0 mg/kg)of MP in early pregnancy had adverse effects on endometrial decidualization function in mice,leading to adverse pregnancy outcome.
作者
田佳林
李伟轲
杨成顺
TIAN Jialin;LI Weike;YANG Chengshun(Thepeoples hospital of Kaizhou district,Chongqing 405400,China;Laboratory of Reproductive Biology,School of Public Health,Chongqing Medical University,Chongqing 400016,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2020年第6期954-961,共8页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:31571190)资助的课题。
