摘要
目的探讨对羟基苯甲酸丙酯对人角质形成细胞(HaCat)的细胞毒作用。方法选用人角质形成细胞株,分别观察不同剂量(3.125～200μg/ml)对羟基苯甲酸丙酯作用不同时间(24～72h)对其细胞毒作用。通过MTT比色法测定其对细胞增殖活力的影响;乳酸脱氢酶(LDH)释放法测定其对细胞膜的损伤。结果对羟基苯甲酸丙酯可引起培养细胞数量明显减少,随作用时间的延长,剂量的增加,对角质形成细胞增殖的抑制作用也显著增大,作用24h在50μg/ml出现细胞增殖抑制,作用48h在25μg/ml出现细胞增殖抑制,而作用72h在6.25μg/ml时就出现细胞增殖抑制,呈现明显的时间-剂量-效应关系;与对照组比较,从50μg/ml起,LDH释放率明显增加,并随剂量的增加而呈上升趋势。结论对羟基苯甲酸丙酯对HaCat细胞具有明显的细胞毒作用,呈现明显的时间-剂量-效应关系。
bjective To know the cytotoxieity of n-propylparaben to HaCat keratinocytes in vitro. Methods HaCat keratinoeytes were exposed to n-propylparaben at different doses（ 3.125-200 μg/ml ） and for different time（24-72 h ）. The effect of n-propylparaben on the proliferation of HaCat keratinocytes was evaluated by MTT assay. Lactate dehydrogenase （LDH） level was detected. Results The decreasing cell viability was observed in n-propylparaben exposure groups at 50 μ/ml for 24 hours, at 25 μg/ml for 48 hours and at 6.25 μ/ml for 72 hours with a dose- time-effect dependent manner. Th~ increasing release rates of LDH were observed among the n-propylparaben exposure groups at the doses of more than 50 μg/ml with a dose-effect dependent manner. Conclusion n-propylparaben can induce cytotoxicity to HaCat keratinocytes in vitro.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2010年第5期420-421,共2页
Journal of Environment and Health
基金
中国疾病预防控制中心环境与健康相关产品安全所青年基金资助项目
