摘要
[目的]构建三叶木通离体快繁体系,进行三叶木通多倍体诱导和鉴定。[方法]以三叶木通带芽茎段为外植体,研究基本培养基和植物激素对其离体培养和再生的影响,构建三叶木通离体快繁体系;采用不同浓度的秋水仙素溶液对增殖芽进行诱导,并用染色体计数法检测材料倍性。[结果]腋芽诱导最适宜培养基为:WPM+1.0 mg/L6-BA+0.1 mg/L NAA+2.0 mg/L GA3,诱导率可达75.12%;适合腋芽增殖的培养基为:WPM+3.0 mg/L 6-BA+0.2 mg/L NAA,增殖系数可达3.71;最佳生根培养基为:1/2 MS+1.0 mg/L NAA+1.0 mg/L GA3,生根率为80%。在多倍体诱导中,增殖芽用0.3%的秋水仙素处理24 h效果最好,多倍体诱导率达15%。[结论]该研究为三叶木通的种质创新及三倍体三叶木通品种的选育奠定了基础和技术支持。
[Objective]The aim of this study was to construct a rapid propagation system of Akebia trifoliata in vitro,and to induce and identify polyploidy of Akebia trifoliata.[Method]In order to establish the tissue culture and rapid propagation system of Akebia trifoliate,the effects of basic culture medium and plant hormones on the in vitro culture and regeneration of the shoot segment of Akebia trifoliata were studied.The proliferation buds were induced by colchicine solution of different concentrations,and the ploidy of the material was detected by chromosome counting.[Result]The results showed that the most suitable medium for axillary bud induction was WPM+1.0 mg/L 6-BA+0.1 mg/L NAA+2.0 mg/L GA3,the induction rate was 75.12%.The suitable medium for axillary bud proliferation was WPM+3.0 mg/L 6-BA+0.2 mg/L NAA,the proliferation coefficient was 3.71.The best rooting medium was 1/2 MS+1.0 mg/L NAA+1.0 mg/L GA3,the rooting rate was 80%.In polyploid induction,the best treatment was 0.3%colchicine for 24 hours,and the polyploid induction rate was 15%.[Conclusion]This study laid a foundation and technical support for germplasm innovation and breeding of triploid of Akebia trifoliata.
作者
姜放军
陶抵辉
JIANG Fang-jun(Hunan Biological and Electromechanical Polytechnic,Changsha,Hunan 410127)
出处
《园艺与种苗》
CAS
2020年第6期1-3,6,共4页
Horticulture & Seed
基金
湖南省教育厅科学研究一般项目课题“三叶木通离体组织细胞染色体加倍研究”(16C0938)。
关键词
三叶木通
带芽茎段
离体快繁
增殖芽
多倍体
Akebia trifoliate
Stems with axillary buds
Rapid propagation in vitro
Proliferation bud
Polyploid
