摘要
以金叶杨(Populus nigra cv.‘Jinye’)带腋芽的茎段为试材,采用了组织培养的方法,研究了金叶杨的腋芽诱导、继代增殖、生根培养,以期为保持其优良性状、实现工厂化育苗提供参考。结果表明:1年生茎段最佳消毒方式是使用0.1%HgCl2溶液处理10 min;最佳诱导培养基为MS培养基;腋芽诱导最佳激素配比为6-BA 0.5 mg·L^-1+NAA 0.3 mg·L^-1,腋芽诱导率为92.6%;增殖效果最佳的激素配比为6-BA 1.0 mg·L^-1+NAA 0.4 mg·L^-1+GA30.2 mg·L^-1,增殖系数为4.4;组培苗生根培养效果最好的培养基为1/2MS+IBA 0.5 mg·L^-1,生根率为91.2%。
The stem segment with axillary buds of Populus nigra cv.‘Jinye’were used as explant,the method of tissue culture was adopted,through the selection of the most suitable culture medium,the axillary bud induction,subculture proliferation and rooting were studied,in order to provide the basic research theory for maintaining its excellent characters and realizing the factory seedling.The results showed that the best disinfection method for annual stems was 0.1%HgCl2 for 10 minutes.For axillary bud induction using stem,the highest induction frequency of axillary bud was 92.6%,using MS containing 6-BA 0.5 mg·L^-1 and NAA 0.3 mg·L^-1.The best hormone ratio for proliferation was 6-BA 1.0 mg·L^-1 and NAA 0.4 mg·L^-1,additional GA30.2 mg·L^-1.On this medium,the multiplication coefficient could reach to 4.4,we inducted seedling rooting on 1/2 MS and 0.5 mg·L^-1 IBA,the rooting rate was 91.2%.
作者
张赫岩
叶冬梅
林涛
彭鹏
华佳文
于晓倩
ZHANG Heyan;YE Dongmei;LIN Tao;PENG Peng;HUA Jiawen;YU Xiaoqian(Forestry College,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010019)
出处
《北方园艺》
CAS
北大核心
2020年第9期97-103,共7页
Northern Horticulture
基金
内蒙古自治区科技计划资助项目(201802109)
内蒙古自治区研究生教育创新计划资助项目(s2018111996z)。
关键词
金叶杨
组织培养
腋芽诱导培养
Populus nigra cv.’Jinye’
tissue culture
axillary bud induction culture
