摘要
以I-101×84K杨为材料,对其组培快繁进行了研究。试验结果表明,MS为合适的基本培养基,根据正交试验结果,诱导I-101×84K杨腋芽增殖的最佳培养基MS+6-BA0.3 mg.L-1+NAA0.3 mg.L-1+蔗糖30 g.L-1+琼脂6 g.L-1;生根培养基以1/2MS+NAA0.02 mg.L-1+蔗糖15 g.L-1+琼脂6 g.L-1为宜,生根试管苗炼苗、移栽后成活率可达90%。对试验过程中的玻璃化问题进行了初步的研究,发现大量元素浓度过高和多次继代可引起试管苗的玻璃化。
Tissue culture for rapid propagation was conducted by using I-101×poplar 84K（Populus alba×（P.alba×P.glandulosa）） stems as the explants.The results showed that the optimal basic medium was the MS.Based on orthogonal experiment,the optimal proliferation medium for the axillary buds was MS＋0.3 mg·L^-16-BA ＋0.3 mg·L^-1 NAA ＋cane sugar 30 g·L^-1＋ agar 0.6 g·L^-1.For rooting,the optimal medium was 1/2MS ＋ 0.02 mg·L^-1 NAA ＋ cane sugar 15 g·L^-1 ＋ agar 0.6 g·L^-1.90% of survival rate of the cultured seedlings were achieved after hardening and transplanting processes.It was found that higher concentrations of macroelements and repeated subculture may cause plantlet vitrification.
出处
《西北林学院学报》
CSCD
北大核心
2010年第5期68-72,共5页
Journal of Northwest Forestry University
基金
国家"十一五"科技支撑计划课题子专题"毛白杨杂交育种与新品种示范"(2006BAD01A1502-2)
西北农林科技大学唐仲英育种基金专项(200905)
