摘要
综述了尺寸排阻色谱、离子交换色谱、亲和色谱和亲水作用色谱对单一聚合度壳寡糖的分离应用,比较和梳理了四种类型的分析色谱柱产品和小试层析柱的实验效果,讨论了其在实际应用中存在的寡糖类型限制和放大可行性等问题,对分离性能更高的混合模式色谱和模拟移动床色谱的潜在应用进行了展望。
Four kinds of chromatographic separation technology applied in chitooligosaccharides with single DP were introduced firstly,including size exclusion chromatography,ion exchange chromatography,affinity chromatography and hydrophilic interaction chromatography.The corresponding commercial analytical column and lab-scale chromatographic column were illustrated and compared,respectively.Additionally,the existed disadvantages like limitation of oligosaccharides and feasibility of scale-up were discussed demonstrably.At the end,the mix-mode chromatography and simulated moving bed chromatography were put forward to make the prospects for future research.
作者
周卫强
沈雪梅
彭超
陈博
卢宗梅
周勇
李义
赵黎明
佟毅
ZHOU Weiqiang;SHEN Xuemei;PENG Chao;CHEN Bo;LU Zongmei;ZHOU Yong;LI Yi;ZHAO Liming;TONG Yi(School of Biotechnology,East China University of Science&Technology,State Key Laboratory of Bioreactor Engineering,Shanghai 200237,China;Nutrition&Health Research Institute,COFCO Corporation,Beijing 102209,China;COFCO Biotechnology Co.,Ltd.,Bengbu 233010,China;National Engineering Research Center of Corn Deep Processing,Changchun 130033,China)
出处
《化学分析计量》
CAS
2020年第4期135-139,共5页
Chemical Analysis And Meterage
关键词
壳寡糖
色谱分离
亲水作用色谱
离子交换色谱
chitooligosaccharides
chromatographic separation
hydrophilic interactions chromatography
ion exchange chromatography
