摘要
目的对不同连作年限附子根际土壤微生物群落及其与土壤化学性质的变化规律进行探讨,以期为附子连作障碍的克服提供理论指导。方法对未种植附子(Y0)和种植1年(Y1)、2年附子(Y2)的土壤采样,应用Illumina MiSeq高通量测序技术和常规分析方法,分析土壤微生物群落和土壤化学性质变化规律。结果获得细菌和真菌总有效序列分别为110218和32819条,在属分类水平上,相较于Y0,Y2部分菌的相对丰度明显增加,如产酸细菌酸杆菌属(Acidobacteriaceae)增加396%,黄单胞菌属(Xanthomonas)增加250%,镰刀菌属(Fusarium)增加17.63%,木霉菌属(Trichoderma)增加7940%,而有益细菌假诺卡菌属(Pseudonocardia)仅为Y0的3.02%,硫杆菌属(Thiobacillus)消失。随着连作年限的增加,土壤中硝态氮、氨态氮、磷酸盐、S和K含量呈现为Y2>Y1>Y0,土壤pH值为Y0>Y1>Y2,Y2的附子产量仅为Y0的64.44%,部分土壤化学性质与附子根际土壤微生物种类显著相关。结论连作会使附子根际土壤微生物群落和土壤化学性质发生改变,根际土壤有益菌减少,致病菌增加可能是导致附子发病率增加,产量减少的原因。
Objective To explore the variations in the structure and chemical properties of soil microbial community in different continuous cropping Aconitum carmichaeli Debx rhizosphere soil,and provide theoretical basis for the control of Aconitum carmichaeli Debx continuous cropping obstacles.Methods Rhizosphere soil samples of 0 year(Y0),1 year(Y1)and 2 years(Y2)continuous cropping Aconitum carmichaeli Debx were collected.Illumina MiSeq high-throughput sequencing technology and relevant analysis method were used to analyze microbial community structure and soil chemical properties.Results A total of 110,218 bacterial operational taxonomic units(OTUs)and 32,819 fungal OTUs were obtained from the 3 soil samples.The proportions of acid-producing bacteria Acidobacteriaceae,Xanthomonas,Fusarium and Trichoderma in Y2 were 396%,250%,17.63%and 7940%higher than those in Y0.The proportion of beneficial bacteria Pseudonocardia in Y2 was only 3.02%of that of Y0.Thiobacillus disappeared from the soil after Aconitum carmichaeli Debx was planted.The contents of NO3-N,NH4+-N,total phosphorus,sulfur and potassium in soil increased along with years with Y2>Y1>Y0,while the soil pH value showed Y0>Y1>Y2.The yield of Y2 was only 64.44%of that of Y1.Several chemical properties of the soil were significantly related to the structure of some microbial communities.Conclusion Aconitum carmichaeli Debx continuous cropping can change the microbial community and chemical properties of rhizosphere soil;the decrease of beneficial microorganisms and increase of pathogenic microorganisms in rhizosphere may lead to the increase of incidence of diseases and yield of Aconitum carmichaeli Debx.
作者
刘世鹏
韦洁敏
李智
江林春
王冬阁
郝学贤
崔浪军
LIU Shipeng;WEI Jiemin;LI Zhi;JIANG Linchun;WANG Dongge;HAO Xuexian;CUI Langjun(Yan′an University,Yan′an,Shaanxi 716000,China)
出处
《中国微生态学杂志》
CAS
CSCD
2020年第5期520-526,共7页
Chinese Journal of Microecology
基金
延安大学引导项目(YDY2017-09)
陕西省教育厅科研项目(14JS114)
科技部科技惠民计划(2012GS610102)
陕西省重点产业创新链(群)项目(2019ZDLSF04-01-0)。
关键词
附子
连作
高通量测序
微生物群落
多样性
Aconitum carmichaeli Debx
Continuous cropping
High-throughput sequencing
Soil microbe community
Diversity
