摘要
为揭示水盐处理下科尔沁沙地土壤细菌群落结构及其对油莎豆(Cyperus esculentus)的影响。以吉林省前郭尔罗斯灌区油莎豆农田为研究对象,开展水、盐双因素(水处理:50%、70%、100%标准灌溉定额;盐处理:无盐胁迫、轻度盐胁迫、中度盐胁迫)随机区组野外控制试验,构建土壤细菌分子生态网络筛选关键菌种,并研究揭示其与油莎豆生长的相互关系。结果表明:(1)油莎豆农田土壤细菌优势菌门为变形菌门(Proteobacteria)(22.85%±3.80%)、酸杆菌门(Acidobacteriota)(20.02%±3.21%)和放线菌门(Actinobacteriota)(18.85%±2.41%)。优势菌属为RB41属、鞘氨醇单胞菌属(Sphingomonas)和红色杆菌属(Rubrobacter)。水盐环境对土壤细菌Alpha多样性无显著影响(P>0.05)。(2)100%标准灌溉定额灌溉处理细菌种间共存关系更强,50%标准灌溉定额灌溉处理细菌种间互作程度、连接紧密度最高。无盐胁迫细菌群落生态网络复杂度、互作程度最高,中度盐胁迫细菌物种之间的共存关系更强。(3)随灌溉量增加,关键菌群数量增加,中度盐胁迫关键菌群数量达到最大。水盐处理下油莎豆土壤关键菌种为红色杆菌属、RB41属、Dongia属、类固醇杆菌属(Steroidobacter)、硝化螺旋菌属(Nitrospira)、鞘氨醇单胞菌属、溶杆菌属和Luteolibacter属。(4)灌溉量变化对油莎豆株高、冠幅、分蘖数、地上干重、羧化酶、脯氨酸和超氧物歧化酶活性具有显著影响(P<0.05);施盐量变化对油莎豆的株高、地上干重、脱落酸、可溶性糖、过氧化物酶活性和丙二醛具有显著影响(P<0.05)。鞘氨醇单胞菌属、硝化螺旋菌属、溶杆菌属、Dongia属、RB41属、类固醇杆菌属和Luteoli⁃bacter属与油莎豆生长生理性状显著相关(P<0.05)。水盐环境改变了土壤细菌群落组成、分子网络及关键菌种,关键菌种则与油莎豆生长生理特征具有显著相关性。研究结果有助于深入揭示水盐生境
To reveal the soil bacterial community structure and its effects on Cyperus esculentus,affected by different irrigation and salinity treatments in the Qian Gorlos Irrigation District,a random plot sampling experiment was conducted using two-factor,three-level(irrigation level:50%,70%,and 100%standard irrigation quota;salt level:control group,mild salinity stress,and moderate salinity stress).Comparative analysis of soil bacterial community characteristics in C.esculentus cropland under different irrigation and salinity treatments was performed.Simultaneously,the molecular ecological network of soil bacteria was constructed to determine the keystone species and its interrelationship with C.esculentus growth.Results showed that(1)The dominant phyla of soil bacteria in C.esculentus cropland were Proteobacteria(22.85%±3.80%),Acidobacteriota(20.02%±3.21%),and Actinobacteriota(18.85%±2.41%).The dominant genera were RB41,Sphingomonas,and Rubrobacter.Bacterial alpha diversity differed insignificantly under different irrigation or salinity treatments(P>0.05).With increasing irrigation,the relative abundance of Proteobacteria gradually increased,whereas that of RB41 gradually decreased.The same trend was observed with increasing salinity stress.(2)The co-existence relationship between bacterial species was stronger in 100%standard irrigation quota treatments,with a positive correlation rate of 78.05%.Additionally,the degree of interactions and tightness of connections between bacterial species was highest at 50%standard irrigation quota treatments.The highest ecological network complexity and degree of interactions among bacterial communities were found in control group soils,and stronger co-existence relationships among bacterial species were found in moderate salinity soils,with a positive correlation rate of 75.31%.(3)The number of keystone species increased with increasing irrigation.Additionally,the RB41 genus appeared under 70%and 100%standard irrigation quota treatments.Significant differences were observed in ke
作者
吴蕊
曹红雨
高广磊
于明含
丁国栋
张英
赵珮杉
WU Rui;CAO Hongyu;GAO Guanglei;YU Minghan;DING Guodong;ZHANG Ying;ZHAO Peishan(School of Soil and Water Conservation,Beijing Forestry University,Engineering Research Centre of Forestry Ecological Engineering,Ministry of Education,Beijing Forestry University,Beijing 100083,China;Yanchi Ecology Research Station of the Mu Us Desert,Yanchi 751500,Ningxia,China;Key Laboratory of State Forestry and Grassland Administration on Soil and Water Conservation,Beijing Forestry University,Beijing 100083,China)
出处
《干旱区研究》
CSCD
北大核心
2023年第12期1938-1948,共11页
Arid Zone Research
基金
国家重点研发计划项目“沙漠化土地油沙豆适沙抗旱耐盐品种选育与适应性机制研究”(2019YFC0507601)。
关键词
水盐处理
细菌群落结构
关键菌种
共现网络
油莎豆
科尔沁沙地
irrigation or salinity treatments
bacterial community structure
keystone species
co-occurrence network
Cyperus esculentus
Horqin Sandy Land
