摘要
【目的】研究白芍根腐病病株和健株土壤根际微生物群落结构变化以及土壤环境因子、微生物群落与白芍根腐病之间的关系,为白芍根腐病的防治提供理论依据。【方法】采用野外调查研究方法,利用Illumina MiSeq高通量测序技术对白芍健株和病株的根际土壤微生物多样性进行测序,并对土壤养分及酶活性进行分析。【结果】与白芍健株相比,病株根际土壤有机质含量显著增加,速效钾含量显著降低,土壤碱性磷酸酶、过氧化物酶显著增加。病株根际土壤细菌丰富度和多样性减少,真菌丰富度和多样性增加。病株根际土壤细菌群落中变形菌门(Proteobacteria)、拟杆菌门(Bacteroidota)、Sumerlaeota的相对丰度显著高于健株,绿弯菌门(Chloroflexi)、放线菌门(Actinobacteriota)、Ethylomirabilota、厚壁菌门(Firmicutes)相对丰度显著下降。鞘氨醇单胞菌属(Sphin⁃gomonas)、Chthoniobacter、Subgroup7、Saccharimonadales在病株根际土壤中的相对丰度高于健康植株。病株根际土壤真菌群落中子囊菌门(Ascomycota)相对丰度增加,担子菌门(Basidiomycota)相对丰度减少,接合菌门(Zygomycota)相对丰度显著高于健株。土壤pH、有机质、有效磷、速效钾、水溶性盐、碱性磷酸酶、过氧化物酶、脲酶、蔗糖酶是影响白芍根际土壤微生物群落变化的重要因子,主要与细菌优势菌门Chloroflexi、Methylomirabilota、Actinobacteriota,真菌优势菌门Chytridiomycota、Basidiomycota、Ascomycota的变化密切相关。【结论】芍药根际土壤中土赤壳属(Ilyonectria)、白粉属(Erysiphe)、镰刀菌属(Fusarium)等有害真菌相对丰度显著增加,养分失衡、酶活性变化是导致白芍根腐病发病的重要原因。
【Objective】Analyzing the changes of microbial community and enzyme activities in the rhizospheric soil of Paeonia lactiflora with root rot and understanding the relationship between soil environmental factors,microbial community and root rot of P.lactiflora would pro⁃vide the scientific basis for controlling root rot of P.lactiflora.【Method】Illumina high throughput sequencing technology and soil physico⁃chemical property method were employed to investigate soil bacterial and fungal communities,soil nutrients and enzyme activities of rhizo⁃sphere soil of healthy and root rot diseased plants of P.lactiflora.【Result】The content of soil organic matter in the rhizosphere of diseased plants significantly increased,the content of available potassium significantly decreased,and the content of soil alkaline phosphatase and peroxidase significantly increased,compared with healthy plants of P.lactiflora.The richness and diversity of bacteria decreased,but the richness and diversity of fungi increased in the rhizosphere soil of root rot diseased plants of P.lactiflora.The relative abundance of Pro⁃teobacteria,Bacteroidota and Sumerlaeota in the rhizosphere soil of root rot diseased plants of P.lactiflora was significantly higher than that of healthy plants,but Chloroflexi,Actinobaciota,Ethylomirabilota and Firmicutes decreased significantly.Sphingomonas,Chtoniobacter,Subgroup7 and Saccharimondales in the rhizosphere soil of root rot diseased plants of P.lactiflora was higher than that of healthy plants.The relative abundance of Ascomycota increased and Basidiomycota decreased in the rhizosphere soil of root rot diseased plants of P.lactiflora,and Zygomycota was significantly higher than that of healthy plants.The pH,organic matter,available phosphorus,available potassium,wa⁃ter⁃soluble salt,alkaline phosphatase,peroxidase,urease and sucrase were important factors that affected the changes of microbial communi⁃ty in the rhizosphere soil of P.lactiflora,which were mainly related to the changes of domi
作者
张英英
吴之涛
杨宪忠
魏玉杰
吴芳
杜雷超
ZHANG Ying-ying;WU Zhi-tao;YANG Xian-zhong;WEI Yu-jie;WU Fang;DU Lei-chao(Gansu Academy of Agri-Engineering Technology,Wuwei,Gansu 733006,China;Key Laboratory of the Special Medicine Source Plant for Germplasm Innovation and Safety Utilization in Gansu Province,Wuwei,Gansu 733006,China;Wuwei Technology Innovation Center of Genuine Medicinal Materials Ecological Cultivation In Qilian Mountain Area,Wuwei,Gansu 733006,China)
出处
《西南农业学报》
CSCD
北大核心
2024年第1期109-118,共10页
Southwest China Journal of Agricultural Sciences
基金
国家中药材产业技术体系(CARS⁃21)
甘肃省技术创新引导计划项目(21CX6NA024)
甘肃武威国家农业科技园区财政专项建设资金项目
武威市科技人才和科学普及计划-自然科学基金项目(WW23A03RPZ010)。
关键词
白芍
土壤微生物群落
根腐病
酶活性
Paeonia lactiflora
Soil microbial community
Root rot
Enzyme activity
