摘要
以种植白及2年的砂质壤土和砂质粘土为试材,采用高通量测序技术和生物信息学分析方法,研究根际真菌群落结构特征,测定土壤理化因子,分析土壤理化因子与真菌群落的相关性,以期为白及根际土壤微生物改良及优质高产栽培提供参考依据。结果表明:共得到43651条真菌基因优化序列,2种根际土壤真菌存在差异,砂质壤土和砂质粘土特有真菌OTU数分别占总数的33.50%、33.60%;α多样性分析表明,砂质壤土中真菌属丰富度高于砂质粘土;群落结构分析表明,除未分类的真菌属之外,镰刀菌属(Fusarium)和被孢霉属(Mortierella)在2种白及根际土壤中均为优势真菌属,砂质壤土中显著富集附球霉属(Epicoccum)、土赤壳属(Ilyonectria)、Knufia,而砂质粘土中显著富集外瓶柄霉属(Exophiala)、原隐球菌属(Saitozyma);土壤有效磷对白及根际土壤真菌群落的α多样性有显著影响,有机质、pH、有效磷、有效镁、有效钙对土壤真菌群落具有显著影响。种植白及的砂质壤土和砂质粘土真菌群落结构存在差异,土壤理化因子对根际真菌具有一定影响,其中有效磷最为显著。
The sandy loam and sandy clay cultivated in Bletilla striata for 2 years were used as test material,the high-throughput sequencing technology and bioinformatics analysis methods were used,the structure of the fungal community in the rhizosphere was studied,soil physical and chemical factors were measured to analyze the correlation between soil physical and chemical factors and fungal community,in order to provide reference for high quality and high yield cultivation of B.striata.The results showed that a total of 43651 optimized fungal gene sequences were obtained,there were differences between the two types of rhizosphere soil fungi,the OTU numbers of unique fungi in sandy loam and sandy clay account for 33.50% and 33.60% of the total,respectively;αdiversity analysis showed that,the richness of fungal genera in sandy loam soil was higher than that in sandy clay;based on community structure analysis,except for unclassified fungal genera,Fusarium and Mortierella were dominant genera in both B.striata rhizosphere soils,Epicocum,Ilyonectria,and Knufia were significantly enriched in sandy loam soil,while Exophiala,Saitozyma was significantly enriched in sandy clay;physical and chemical factors affect the fungal community in the rhizosphere soil of B.striata,effects of soil available phosphorus on rhizosphere soil of B.striata fungal communitiesαdiversity had a significant impact,pH,available phosphorus,available magnesium and available calcium had a significant impact on soil fungal communities.There were differences in the fungal community structure between sandy loam soil and sandy clay soil planted with B.striata,soil physicochemical factors had a certain impact on rhizosphere fungi,among them,available phosphorus was the most significant.
作者
许清清
付艳
周涛
张进强
张永萍
肖承鸿
XU Qingqing;FU Yan;ZHOU Tao;ZHANG Jinqiang;ZHANG Yongping;XIAO Chenghong(Institute of Traditional Chinese Medicine and Ethnic Medicine Resources,Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550025)
出处
《北方园艺》
CAS
北大核心
2024年第2期79-85,共7页
Northern Horticulture
基金
国家自然科学基金委-贵州省人民政府联合基金资助项目(U1812403)
贵州省高层次创新型人才资助项目(黔科合平台人才[2018]5638-2)
贵州省高等学校分子生药学重点实验室资助项目(黔教技[2022]021号)
中央本级重大增减支资助项目(2060302)
现代农业产业技术体系建设专项资助项目(CARS-21)。
关键词
白及
根际土壤
真菌群落
理化因子
相关性
Bletilla striata
rhizosphere soil
fungal community
physical and chemical factors
correlation
