摘要
目的探究前列环素E1(prostacyclin E1,PGE1)对肝动脉碘化油栓塞术后肝纤维化家兔Smad2和Smad4 mRNA表达水平的影响。方法选取成年家兔36只,采用随机数字表法分为假手术组、模型组和给药组。饲养1周后3组兔均在麻醉下行气管插管,并模拟行肝动脉碘化油栓塞术,然后假手术组经肝右动脉注入生理盐水1 mL,给药组经肝右动脉注入碘化油1 mL+0.2 mL PGE1,模型组经肝右动脉注入碘化油1 mL。术后2、4、6和8 d分别用酶联免疫吸附试验检测各组兔血清学指标,术后8 d解剖兔并取其肝组织作病理学检查,并且采用RT-PCR法检测各组兔肝组织中Smad2和Smad4 mRNA水平。结果假手术组兔术后2、4、6和8 d的血清学指标无明显改变。与假手术组相比,给药组和模型组各时间点的血清学指标随时间增加而明显升高(P<0.05);给药组各时间点血清学指标较模型组明显下降(P<0.05)。假手术组兔术后8 d肝病理学检查无明显纤维化发生;而给药组和模型组兔病理学标本均发生不同程度的肝纤维化,且给药组较模型组肝纤维化明显(P<0.05)。给药组和模型组较假手术组兔肝组织中Smad2和Smad4 mRNA的表达水平明显升高(P<0.05),且给药组较模型组肝组织中Smad2和Smad4 mRNA表达水平明显降低(P<0.05)。结论PGE1应用于肝动脉碘化油栓塞术,能改善术后肝纤维化情况,并降低肝组织中Smad2和Smad4 mRNA表达水平,从而对肝损伤保护有一定的积极作用。
Objective To investigate the effect of prostacyclin E1(PGE1)on the expressions of Smad2 and Smad4 mRNA in rabbits with hepatic fibrosis after embolization with lipiodol.Methods Thirty-six adult rabbits were randomly divided into sham operation group,model group and administration group.After one week acclimation,all the rabbits in the three groups were intubated under anesthesia,and hepatic artery embolization with iodized oil was simulated.Then,1 mL normal saline was injected into the right hepatic artery in the sham operation group,1 mL iodized oil and 0.2 mL PGE1 were injected into the right hepatic artery in the administration group,and 1 mL iodized oil was injected through the right hepatic artery in the model group.On the 2nd,4th,6th and 8th day after the operation,the serum indices of each group were detected by ELISA.On the 8 th day after the operation,all the rabbits were euthanized,and their liver tissues were taken out for pathological examination.The mRNA levels of Smad2 and Smad4 in the liver tissues of each group were detected by RT-PCR.Results There was no significant change in the serological indices of the rabbits in the sham operation group on the 2nd,4th,6th and 8th day after operation.Compared with the sham operation group,the serological indices of the administration group and the model group increased significantly with time(P<0.05).Compared with the model group,the serological indices of each time point in the administration group decreased significantly(P<0.05).On the 8th day after operation,there was no obvious fibrosis in the sham operation group,while there were different degrees of fibrosis in the pathological specimens of the administration group and the model group,and the fibrosis in the administration group was more obvious than that in the model group(P<0.05).The expression levels of Smad2 and Smad4 mRNA in the liver tissues of rabbits in the administration group and the model group were significantly higher than those in the sham operation group(P<0.05).The expressions of Smad2 a
作者
李磊
李向利
胡惠敏
LI Lei;LI Xiangli;HU Huimin(Department of Hepatobiliary Surgery,Suzhou Xiangcheng People's Hospital,Suzhou 215131,China;Department of General Surgery,Suzhou Xiangcheng People's Hospital,Suzhou 215131,China;Department of Pathology,Suzhou Xiangcheng People's Hospital,Suzhou 215131,China)
出处
《实验动物与比较医学》
CAS
2020年第3期212-217,共6页
Laboratory Animal and Comparative Medicine
基金
苏州市科技计划项目(SYS201658)。
