摘要
目的确认湖南省首起输入性基孔肯雅热(Chikungunya fever,CHIK)疫情。方法采集病例和同行人员的血清标本,采用实时荧光定量PCR方法进行登革病毒(Dengue virus,DENV)、黄热病毒(Yellow fever virus,YFV)、基孔肯雅病毒(Chikungunya virus,CHIKV)核酸检测,对核酸检测阳性病毒标本进行基因序列测定,对测序结果进行生物信息学分析,构建系统进化树。结果病例血清标本CHIKV核酸阳性,5名同行人员中有1人血清标本CHIKV核酸阳性。基因序列Blast比对显示与CHIKV序列同源性>99%。系统进化树显示感染CHIKV属于ECSA基因型。结论通过流行病学调查和病原学检测,确定了湖南省首起输入性CHIK疫情。
Objective To confirm the first imported Chikungunya fever(CHIK)epidemic in Hunan province.Methods Serum samples of patients and colleagues were collected.The nucleic acids of Dengue virus(DENV),Yellow fever virus(YFV),Chikungunya virus(CHIKV)were detected by real-time fluorescent quantitative PCR.The positive PCR products were sequenced.Phylogenetic tree was constructed.Results The serum samples of the patient and one of the five colleagues were positive for CHIKV.The Blast comparison of gene sequence showed 99%homology with CHIKV sequences.The infected CHIKV belonged to ECSA genotype in the phylogenetic tree.Conclusions The first imported CHIK epidemic in Hunan province was confirmed through the epidemiological survey and etiologic detection.
作者
何方玲
张恒娇
王娟
何舰
龙绪湘
刘蓓
杨锐
胡世雄
湛志飞
蔡亮
高立冬
He Fangling;Zhang Hengjiao;Wang Juan;He Jian;Long Xuxiang;Liu Bei;Yang Rui;Hu Shixiong;Zhan Zhifei;Cai Liang;Gao Lidong(Hunan Provincial Center for Disease Control and Prevention,Changsha 410005,China;Huaihua Center for Disease Control and Prevention,Huaihua 418000,China;Hecheng Center for Disease Control and Prevention,Huaihua 418000,China;Zhijiang Center for Disease Control and Prevention,Zhijiang 419100,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2020年第2期175-179,共5页
Chinese Journal of Experimental and Clinical Virology
基金
国家科技重大专项(2018ZX10713002-001-003)
湖南省卫生厅重点课题(A2011-006)。
