摘要
目的对福州市2004年登革热疫情及病原学特征进行分析。方法调查和分析福州市2004年登革热流行病学特点及影响因素;用C6／36细胞分离患者血清标本中病毒,并用单克隆抗体鉴定型别;通过比对核苷酸序列,分析本次疫情可能的传染源。结果福州市2004年9月中旬起至10月底发生一起登革热暴发疫情,所确认的93例病例消长情况基本与当地的蚊媒消长情况相一致。从病例的10份血清标本中分离出6株病毒,经单克隆抗体鉴定均为登革热病毒1型,病毒基因的核苷酸序列比对证明同源性与东埔寨分离株(DENV-1／KHM／2001;GenBank Accession No．L0904278)最为接近。结论福州市2004年登革热疫情为感染登革1型病毒所引起;疫情的发生主要为输入性病例流行引起。
Objective To study the epidemiology and etiologic characteristics of a Dengue fever outbreak in Fuzhou from the beginning of September to the end of October in 2004 in order to understand the source of infection. Methods Data on descriptive epidemiology was collected to study the characteristics and related factors to the epidemic. Dengue virus was isolated through the use of C6/36 cell line while viral serotypes were identified by indirect immunofluorecent assay with type-specific monoclonal antibody. The sources of infection were traced by nucleotide sequencing. Results During the epidemic, 93 cases occured consistently with the region entomoplily growth and decay. The viruses of 6 strains isolated from 10 patients＇ blood specimens were identified as dengue virus type 1. Phylogenetic evidence suggested that the viral isolate had high genetic relation with the isolates from Kampuchea（DENV-1/KHM/2001; GenBank Accession No. L0904278 ）. Conclusion The epidemic was caused by introduction of patients migrating into Fuzhou.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2006年第5期371-374,共4页
Chinese Journal of Epidemiology
基金
福建省重点专项基金资助项目(2004YZ01-1)
关键词
登革热1型病毒
疫情
序列分析
Dengue virus type 1
Epidemic
Nucleotide sequencing
