摘要
【目的】研究不同生长调节剂处理对金叶银杏硬枝扦插生根率和成活率的影响,探讨金叶银杏的生根机制,为金叶银杏苗木扦插快繁技术体系的建立提供技术支持和理论指导。【方法】通过NAA和IBA处理金叶银杏硬枝插穗,检测它们对插穗的生根状态、生根指标及IBA对扦插过程中插穗皮部可溶性糖、抗氧化酶、激素含量的影响,探究金叶银杏硬枝扦插繁殖技术和生根机理。【结果】1)金叶银杏插穗愈伤组织诱导期和形成期、不定根发生期和形成期分别在插后的20~26、26~42、42~53、53~79 d。2)800 mg·L^-1 IBA和400 mg·L^-1 NAA+400 mg·L^-1IBA(混合)处理的插穗愈伤组织和不定根出现期及生根率与根数都较其他相应处理的效果好,其中生根率分别达64.4%和51.1%;不定根数分别达8.6条和9.3条。3)插穗生根过程中(500 mg·L^-1 IBA处理)基部韧皮部的SOD、PPO活性在插后40、60 d达到高峰,均比对照提前20 d达到峰值;插穗的POD活性出现上升-下降-升高的趋势,插穗可溶性糖也出现升高-下降-升高的趋势,但时间点不一致;而淀粉的含量出现下降-上升的趋势。4)生根期间插穗基部韧皮部的IAA含量出现了2个峰值和一个谷值;而ZR含量在第40天达到峰值后下降;GA含量先下降后上升;ABA含量在插后的前期高后期低,且值都低于同期对照。IAA/ABA比值在插后的第40天(愈伤组织形成期)达到谷底,后又上升(不定根形成期)。IAA/GA在插后的40~60 d内快速上升,80 d后又开始大幅下降;IAA/ZR值在扦插愈伤组织形成期(40 d)呈下降趋势,到60 d时(不定根发生期)又急剧上升到峰值。【结论】高活性的SOD和POD有利于插穗初期愈伤组织的形成(40 d),高活性的POD与PPO有利于不定根的形成(60 d)。较高的IAA含量是保证愈伤组织形成(20~40 d)的必要条件,不定根形成期(60 d)需要较高的IAA/ZR、IAA/ABA、IAA/GA比值。本研究为金叶银杏扦插繁殖技术和
【Objective】Ginkgo biloba ‘Golden-leaf’ is a new variety species of ginkgo with golden-yellow leaves in two seasons including spring and autumn, which selected from bud mutation, and has an important ornamental value and market prospect. Cutting propagation could maintain its good genetic traints, and breed a mass of seedlings to meet the market demand.【Method】In this study, the changes of rooting process,rooting indexes, the soluble sugar, starch and endogenous hormones contents, and the SOD, POD, PPO activities in hard-wood cutting of the Ginkgo biloba ‘Golden-leaf’ were investigated by the cuttings soaked into the different doses of NAA(α-Naphthylacetic acid) and IBA(indole-3-butyric acid) for 1 h before cutting.【Result】1) The callus induction and formation stages, and adventitious root generation and formation stages of ‘Golden-leaf’ ginkgo cuttings were observed at 20-26 d, 26-42 d, 42-53 d and 53-79 d during the cutting rooting process, respectively. 2) The optimal effects such as callus and adventitious root appearance stages, rooting rates and number of roots were observed in 800 mg·L^-1 IBA, or 400 mg·L^-1 NAA+ 400 mg·L^-1 IBA(mixed) treatments, and the rooting rates were attained to 64.4% and 51.1%;the number of roots were 8.6 and 9.3, respectively. 3) During the rooting process of cuttings(treated by 500 mg·L^-1 IBA), the activities of SOD(superoxide dismutase) and PPO(polyphenol oxidase) in the basal phloem of cuttings showed a same trend of increasing first and then decreasing, both reached a peak earlier 20 days than the control. POD(peroxidase) activity was observed a upward-downward-upward trend. The same trend was observed in soluble sugar content. However, the change of starch content was opposite to that of soluble sugar, which decreased significantly within 40 days and gradually increased after 60 days. 4) During the rooting period, IAA(3-indoleacetic acid) content in the basal phloem of cuttings showed two peaks and a bottom, however, the IAA content in the control
作者
王瑞敏
祝凌云
陈颖
姚秀文
白景伟
陈旭
张齐齐
曹福亮
WANG Ruimin;ZHU Lingyun;CHEN Ying;YAO Xiuwen;BAI Jingwei;CHEN Xu;ZHANG Qiqi;CAO Fuliang(Con-Innovation Center for the Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;College of Forestry,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;Nanjing Angkeli Pharmaceutical Technology Innovation Research Institute Co.,Ltd.,Nanjing 211100,Jiangsu,China)
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2020年第5期28-37,共10页
Journal of Central South University of Forestry & Technology
基金
国家“十三五”重点研发计划课题(2017YFD0601301)
国家自科学基金项目(31770674)
江苏省高校优势学科建设工程资助项目(PAPD)
南京林业大学大学生创新训练计划项目(2017NFUSPITP032)。
关键词
金叶银杏
硬枝扦插
IBA处理
植物激素
生根机理
golden-leaf ginkgo
hard-wood cutting
IBA treatment
phytohormones
rooting mechanism
