摘要
由β-淀粉样蛋白(Aβ)生成的Aβ斑块是阿尔兹海默症(AD)产生的重要病理特征之一。在阿尔兹海默症治疗的相关研究中,实现对Aβ斑块的有效检测和降解是需要解决的重要科学问题。由于Aβ单体聚集成斑块后具有较强的自体荧光,因此本研究利用非线性光学成像方法实现对Aβ斑块的无标记成像,同时利用光敏剂的光动力效应对Aβ斑块进行降解。比较了不同浓度下光敏剂对Aβ斑块的降解效果,制备了相应的脂质体并成功地将其用于对Aβ斑块的降解。探讨了无标记光学成像及光动力疗法在阿尔兹海默症研究中的潜在应用价值,为优化阿尔兹海默症的诊断和治疗提供了新的途径。
Formation ofβ-Amyloid(Aβ)plaques is one of the most significant pathological features of Alzheimer’s disease(AD).Detection and degradation of Aβplaques are crucial in AD treatment.When Aβmonomers aggregate to form plaques,they produce strong autofluorescence.In this study,we investigate label-free imaging of Aβplaques by using the nonlinear optical imaging method and study their degradation via photodynamic effect of the photosensitizer.Moreover,we study the relationship between photosensitizers with different concentrations and degradation effect of Aβplaques.Corresponding liposomes are prepared and they are applied to the degradation of Aβplaques.The potential applications of label-free optical imaging and photodynamic therapy in AD research are discussed,and a new way for optimizing AD diagnosis and treatment is provided.
作者
黄燕霞
许皓
栾萍
Ohulchanskyy Tymish Y
屈军乐
Huang Yanxia;Xu Hao;Luan Ping;Ohulchanskyy Tymish Y;Qu Junle(Guangdong Province Key Laboratory of Optoelectronic Devices and Systems,Ministry of Education,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,China;Health Science Center,Shenzhen University,Shenzhen 518060,China)
出处
《中国激光》
EI
CAS
CSCD
北大核心
2020年第2期332-340,共9页
Chinese Journal of Lasers
基金
国家自然科学基金(61525503,61620106016,61835009,61705139)
广东省高等学校科技创新(重点)项目(2015KGJHZ002,2016KCXTD007)
广东省自然科学基金(2014A030312008)
深圳市基础研究项目(JCYJ2017041210500352090620324,JCYJ20170817094735945)。
