摘要
探讨了新型卟啉类光敏剂PA1介导的光动力抗菌(PACT)联合抗生素治疗重度创伤感染大鼠的效果并简要探讨其机制。健康雄性Wistar大白鼠24只(清洁级)作为实验对象,建立大面积创伤感染模型,随机分成模型对照组、PACT治疗组、抗生素治疗组、PACT联合抗生素治疗组,每组6只,共治疗8d。检测不同时间点各组创面下的菌落数和创面愈合率,并采用酶联免疫吸附测定(ELISA)方法检测创面周边组织碱性成纤维因子(bFGF)、肿瘤坏死因子α(TNF-α)和白介素6(IL-6)的表达。结果表明PACT联合抗生素治疗与模型对照组相比能显著减轻大鼠创伤组织的炎症,减少创面组织中细菌的数量,加快创面愈合速率,缩短创面愈合时间;同时还能提高创面组织中bFGF的含量,抑制组织中炎症相关因子TNF-α和IL-6的表达,其效果优于单用PACT的治疗组和单用抗生素的治疗组。结果表明PACT联合抗生素可以治疗大面积创伤感染。
To investigate the treatment effect of new porphyrin photosensitizer PAl mediated photodynamic antimicrobial chemotherapy （PACT） combined with antibiotics on the rat model with severe massive traumatic infection and the mechanism of the combination treatment, 24 healthy male （clean grade） Wistar rats were used to create the massive trauma infection model. Rats were randomly divided into four groups： model control group, PACT group, antibiotic treatment group and PACT＋antibiotic treatment group. There were six rats in each group. The wound healing rate and the number of bacterial colonies were detected at different time points in each experimental group during 8 d treatment. Enzyme-linked immunosorbent assay （ELISA） method was used to detect the expression of basic fibroblast growth factor （bFGF）, tumor necrosis factor a （TNF-a） and interleukin-6 （IL-6） in the peripheral tissues of the wound. The results show that PACT combined with antibiotic therapy can significantly reduce inflammatory reaction and bacterial colonies in rat trauma tissues, accelerate the wound healing, shorten the wound healing time, increase the content of basic fibroblast factor, and inhibit the expression of inflammatory cytokines TNF-a and IL-6. The treatment effect of PACT combined with antibiotic therapy is better than antibiotic therapy or PACT therapy alone. It suggests that PACT combined with antibiotics can treat large area traumatic infection.
出处
《激光与光电子学进展》
CSCD
北大核心
2017年第3期223-229,共7页
Laser & Optoelectronics Progress
基金
天津市重大科技支撑项目(12ZCDZSY11900)
河北大学医学学科建设项目(2015A2001)
河北大学大学生创新训练计划(2016149)
关键词
医用光学
光动力抗菌疗法
创伤感染
光敏剂
卟啉衍生物
medical optics
photodynamic antimicrobial therapy
traumatic infection
photosensitizer
porphyrin derivative
