摘要
目的探讨湿润暴露疗法/湿润烧伤膏(MEBT/MEBO)治疗慢性难愈合创面的超微病理及丝裂原活化蛋白激酶激酶(MAPKK)、c-myc mRNA表达的机制。方法选取SPF级雄性SD大鼠80只,采用随机数字表法将其分为空白对照组、模型组、湿润烧伤膏组、康复新液组,每组20只。空白对照组建立全层皮肤缺损开放性创面,模型组、湿润烧伤膏组、康复新液组建立全层皮肤难愈合创面。空白对照组建立创面后不做其他处理,模型组外敷0.9%氯化钠溶液,湿润烧伤膏组外敷MEBO,康复新液组外喷康复新液。在用药后第3、6、12天利用透射电镜观察大鼠皮肤创面细胞超微结构;用药后第12天进行荧光定量聚合酶链反应(RT-PCR),分析MAPKK、c-myc mRNA的表达水平。结果用药后第3、6、12天,湿润烧伤膏组与康复新液组细胞结构逐步改善,各细胞器形态结构逐渐恢复至正常水平。4组MAPKK、c-myc mRNA表达水平比较,差异均有统计学意义(P<0.05)。模型组、湿润烧伤膏组与康复新液组较空白对照组MAPKK、c-myc mRNA表达水平降低,湿润烧伤膏组、康复新液组较模型组MAPKK、c-myc mRNA表达水平均增高(P<0.05);湿润烧伤膏组与康复新液组MAPKK、c-myc mRNA表达水平比较,差异无统计学意义(P>0.05)。结论 MEBT/MEBO能够改善细胞超微病理结构,提高MAPKK、c-myc mRNA的表达水平,促进慢性难愈合创面恢复。
Objective To study the ultrastructural pathology and the mechanism of expression of MAPKK mRNA ＆amp; c- myc mRNA of chronic refractory ulcer wound treated by MEBT/ MEBO. Methods A total of 80 SPF male SD rats were randomly divided into four groups by random number table method：blank control group,model group,MEBO ointment group and new healing lotion group,20 rats in each group. The full - thickness skin defect open wound was established among rats in blank control group,full - thickness skin refractory ulcer wound was established among rats in model group,MEBO ointment group and new healing lotion group. After the establishment of wound,rats in blank control group were not managed,rats in model group were dressed with normal saline,rats in MEBO ointment group were dressed with MEBO,rats in new healing lotion group were sprayed with new healing lotion. 3,6,12 days after treatment,the cellular ultrastructural structure of the skin wound was observed using TEM microscope. 12 days after treatment,the expression levels of MAPKK mRNA and c - myc mRNA using RT - PCR technology. Results 3,6 and 12 days after treatment,the cellular ultrastructural structure of the skin wound among rats in MEBO ointment group and new healing lotion group gradually improved,morphology and structure of cell organs restored to normal. There were significant differences in expression levels of MAPKK mRNA and c - myc mRNA among four groups（ P ﹤ 0. 05）. The expression levels of MAPKK mRNA and c - myc mRNA in model group,MEBO ointment group and new healing lotion group were significantly lower than those in blank control group,respectively;while the expression levels of MAPKK mRNA and c - myc mRNA in MEBO ointment group and new healing lotion group were significantly higher than those in model group,respectively（P ﹤0. 05）. There was no significant difference in expression levels of MAPKK mRNA and c - myc mRNA between MEBO ointment group and new healing lotion group（ P ﹥ 0. 05）. Conclusion MEBT/ MEBO can improve cellular pat
出处
《中国全科医学》
CAS
CSCD
北大核心
2015年第3期294-299,共6页
Chinese General Practice
基金
国家自然科学基金资助项目(81360547)
广西自然科学基金资助项目(2013GXNSFDA019020)
