摘要
棉花纤维是重要的天然纺织材料,是最长的单细胞,是研究纤维发育的良好材料。本研究以亚洲棉短绒突变体(FZ)及其野生型(fz)为材料,结合扫描电镜、石蜡切片、RNA-seq技术,解析棉花短绒起始的可能机制。与野生型(fz)的0DPA时期胚珠相比,突变体的胚珠在该时期仅有少量的纤维起始。在+3DPA时,突变体没有短绒细胞起始,仅有长纤维细胞,而野生型有大量的短纤维细胞和长纤维细胞。对这2个材料的0DPA、+3DPA、+5DPA和+8DPA胚珠差异基因分析结果显示,在短绒突变体(FZ)和野生型(fz)的4个纤维发育时期共挖掘出3780个差异表达基因,其中0DPA时差异基因数目最少,随着胚珠发育时间的延长,差异基因的数目逐渐增加。KEGG分析发现这些基因主要参与蜡质、角质生物合成,以及苯丙烷代谢和植物信号传导过程。共表达趋势分析显示,在突变体+3DPA上调的差异基因中,参与离子结合、MAPK级联反应、氧化还原活性和转录调控的基因表达受到正影响(表达水平提高),造成突变体短绒纤维不能正常起始。这些结果描述了二倍体亚洲棉短绒起始的动态变化,可为进一步研究棉纤维发育提供参考。
Cotton fiber,one of main nature textile and longest single cell,is a good material for researching the cotton fiber development.In this study,fuzz mutant(FZ)and wild-type(fz)which belong to diploid Gossypium arboreum species were used for unraveling molecular mechanism of fuzz fiber initiation by scanning electron microscopy(SEM),paraffin section and RNA sequencing technology.It was found that any fibers scarcely initiated on the ovule surface of the mutant at 0DPA,compared with that of the wild-type.Only lint fiber cells emerged on the ovule surface of fuzz mutant,while abundant fuzz and lint fiber cells appeared in wild-type at+3DPA stage.The transcriptome analysis of FZ and fz across the four fiber development stages(0DPA,+3DPA,+5DPA,and+8DPA)indicated that the number of differentially expressed gene was 3780.There were litter differentially expressed genes at 0DPA in FZ and fz.In the proceed of ovule development,the number of differentially expressed genes was increased gradually.The KEGG enriched pathway analysis illustrated that these differentially expressed genes were involved in cutin,suberine and wax biosynthesis,phenylalanine metabolism pathways and plant hormone signal transduction pathways.The analysis of co-expressed gene trend lines indicated that the up-regulated genes in mutant at+3DPA were all involved in metal ion binding,MAPK cascade,oxidoreductase activity and transcription regulation,resulting in failed fuzz fiber cells initiation in mutant.These findings elaborated a dynamic variation about fuzz fiber initiation in diploid G.arboreum,which provides a reference for further research in the cotton fiber development.
作者
王晓阳
王丽媛
潘兆娥
何守朴
王骁
龚文芳
杜雄明
WANG Xiao-Yang;WANG Li-Yuan;PAN Zhao-E;HE Shou-Pu;WANG Xiao;GONG Wen-Fang;DU Xiong-Ming(Institute of Cotton Research,Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology,Anyang 455000,Henan,China;Zhengzhou Research Base,State Key Laboratory of Cotton Biology/Zhengzhou University,Zhengzhou 450001,Henan,China;Key Laboratory of Cultivation and Protection for Non-wood Forest,Ministry of Education/Central South University of Forestry and Technology,Changsha 410004,Hunan,China)
出处
《作物学报》
CAS
CSCD
北大核心
2020年第5期645-660,共16页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31601353)
国家重点研发计划项目(2017YFD0101601-03)资助。
关键词
亚洲棉
短绒突变体
差异表达基因
共表达趋势
Gossypium arboreum
fuzzless mutant
differentially expressed gene
co-expressed trend
