摘要
DELLA是赤霉素信号传导途径中一类重要的阻遏蛋白。本研究通过RT-PCR技术,从橡胶树优良品种‘热研7-33-97’胶乳中克隆了1个DELLA蛋白编码基因(GenBank登录号为KT696440)。该基因全长cDNA序列2135bp,阅读框1905bp,编码634个氨基酸,其编码蛋白含有DELLA和GRAS结构域,分子量为69.89ku,理论等电点为5.50。HbGAIP-B氨基酸序列与麻疯树JcGAIP-B、蓖麻RcGAIP-B、拟南芥AtRGA、AtGAI、AtRGL1、AtRGL2和AtRGL3的相似性分别为82.43%、78.18%、65.05%、61.73%、52.28%、53.51%和49.92%。进化树分析表明,HbGAIP-B与JcGAIP-B亲缘关系最近。定量PCR分析表明,HbGAIP-B的表达受割胶处理诱导下调。赤霉素和乙烯利处理4h显著上调HbGAIP-B的表达,茉莉酸甲酯处理4h小幅下调HbGAIP-B表达。
DELLA are kind of repressor proteins of GA signaling. In this work, a full length cDNA sequence of HbGAIP-B （GenBank accession no. KT696440） was obtained from the latex of rubber tree clone ＇CATAS7-33-97＇ by RT-PCR. HbGAIP-B was 2 135 base pairs （bp） in length and contained an open reading frame （ORF） of 1 642 bp. The ORF encoded 613 amino acid residues, which contained DELLA and GRAS domain, with a predicted molecular mass of 66.476 ku and a pI of 5.19. The deduced amino acid sequences of HbGAIP-B showed high identities of 82.43%, 78.18%, 65.05%, 61.73%, 52.28%, 53.51% and 49.92% to JcGAIP-B, RcGAIP-B, AtRGA, AtGAI, AtRGL1, AtRGL2 and AtRGL3, respectively. Real-time quantitative PCR analysis in the latex showed that, compared with the control, HbGAIP-B transcript levels were significantly down-regulated in latex by consecutive tapping. The transcript levels were significantly up-regulated at 4 hours by gibberellins and ethephon. And methyl jasmonate reduced slightly the expression of HbGAIP-B.
出处
《热带作物学报》
CSCD
北大核心
2016年第5期881-887,共7页
Chinese Journal of Tropical Crops
基金
国家天然橡胶产业技术体系(No.CARS-34-GW1)
国家自然科学基金(No.31300504)
