摘要
为进一步揭示苹果(Malus×domestica)中GA信号转导机制以及MdDELLAs的生理功能,以‘嘎拉’苹果为试材,克隆基因MdRGL3a(GenBank:DQ007887),并通过在拟南芥中过量表达,分析该基因的功能。qRT-PCR结果表明,MdRGL3a在苹果不同组织中都有表达,其中在花和果实中表达量较高。在拟南芥中异位表达MdRGL3a,表现为根长和下胚轴变短,种子萌发较慢,叶片变小,植株矮化。过表达MdRGL3a拟南芥植株对赤霉素介导的生长反应敏感性降低,证明MdRGL3a是GA信号途径中的一个负调控因子。
In order to further reveal the mechanism of GA signal transduction in apple（Malus × domestica） and the physiological function of MdDELLAs,a gene named MdRGL3a（Gen Bank：DQ007887）cloned from ＇Royal Gala＇ was analyzed. The function of this gene was analyzed by ectopic expression of MdRGL3 a in Arabidopsis thaliana. Real-time quantitative PCR results showed that MdRGL3 a was expressed in different tissues,and the expression level was higher in flowers and fruits. Ectopic expression of MdRGL3 a in Arabidopsis thaliana reduced response to GA3,which exhibited shorter root and hypocotyl,slower germination,smaller leaves and shorter stalk. The result of Arabidopsis thaliana plants treated with GA3 showed that MdRGL3 a reduced the promotion of GA for plants growth and development,revealing that MdRGL3 a might be a negative regulator of GA signaling pathway.
出处
《园艺学报》
CAS
CSCD
北大核心
2017年第6期1021-1028,共8页
Acta Horticulturae Sinica
基金
农业部公益性行业科研专项(201203075-3)
教育部“创新团队发展计划”项目(IRT15R42)
山东省现代农业产业技术体系创新团队项目(SDAIT-06-03)
