摘要
通过转录组测序筛选草棉同源多倍体与二倍体相比差异表达基因,为同源多倍体的表型变化及其染色体加倍过程中遗传物质的变化提供基因表达水平的依据。本研究以草棉二倍体(CK)和同源多倍体后代为材料,通过流式细胞仪鉴定出同源三倍体(T)和同源四倍体(M)植株,比较同源四倍体与二倍体的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、脯氨酸和可溶性蛋白含量生理指标,并进行转录组测序分析。草棉同源四倍体叶片的SOD、POD、CAT、脯氨酸和可溶性蛋白含量均显著高于二倍体。DESeq差异分析结果表明, CK vs T、T vs M、CK vs M分别有4166 (2832个上调, 1334个下调)、4037 (1766个上调, 2271个下调)、4184 (2679个上调, 1505个下调)个DEGs,有205个DEGs是不同倍性植株两两比较的3组间共同表达的。GO功能分析表明, DEGs在膜的固有成分、膜的组成部分、催化活性等途径差异显著。KEGG富集分析表明, DEGs在类黄酮生物合成、苯丙烷生物合成、光合作用-天线蛋白等通路较为活跃。通过富集分析筛选CK vs T和CK vs M中与倍性相关的DEGs发现,多倍体在应激反应(62.46%和54.84%)、逆境反应(57.42%和53.85%)、细胞(70.16%和66.22%)、代谢(72.73%和66.14%)、生物活性物质和抗性(60.33%和63.37%)、光合(58.06%和63.16%)等方面的差异基因大都是上调表达,这可能是导致多倍体优势的关键因子。Nr注释信息CK vs M中共获得27个与抗性相关的基因(19个上调, 8个下调), 11个与光合相关的基因(9个上调,2个下调)。对不同倍性草棉与光合相关的27个DEGs进行聚类分析发现, 18个(66.67%)基因在四倍体中表达丰度最高。对3组间共同表达的205个DEGs进行Nr注释和表达情况分析发现,有30个基因都表现为上调表达,且在四倍体中表达量最高,7个基因都表现为下调表达,且在四倍体中表达量最低,这37个基因可能是受DNA剂量影响或是与
To investigate the basis for the relative expression level of genes between the phenotypic changes of autopolyploid and the changes of genetic material during chromosome doubling,the differentially expressed genes between autopolyploids and dip-loids were screened by transcriptome sequencing of G.herbaceum.In this study,the diploid G.herbaceum(CK)and autopolyploid progenies were used as materials,autotriploid(T)and autotetraploid(M)plants were identified by flow cytometry.After the physiological indexes of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),proline,and soluble protein contents of autotetraploids and diploids were compared,and transcriptome sequencing were analyzed.The contents of SOD,POD,CAT,proline,and soluble protein in the leaves of autotetraploids were significantly higher than those of diploids.DESeq difference analysis showed that there were 4166(2832 up-regulated and 1334 down-regulated),4037(1766 up-regulated and 2271 down-regulated),and 4184(2679 up-regulated and 1505 down-regulated)DEGs,in CK vs T,T vs M,and CK vs M,respectively,and 205 DEGs were common expressed among three groups of pairwise comparison of different ploidy plants.GO function re-vealed that DEGs had significant differences in intrinsic component of membrane,integral component of membrane,catalytic activity.KEGG enrichment indicated that DEGs was more active than other pathways in flavonoid biosynthesis,phenylpropane biosynthesis,and photosynthesis-antenna protein.Through enrichment analysis,the DEGs related to ploidy in CK vs T and CK vs M were screened,and the results demonstrated that polyploidy were detected mostly up-regulated,in response to stimulus(62.46%and 54.84%),stress(57.42%and 53.85%),cell(70.16%and 66.22%),metabolism(72.73%and 66.14%),biologically active substances and resistance(60.33%and 63.37%),photosynthesis(58.06%and 63.16%),which may be the key factor lead-ing to polyploid advantage.A total of 27 resistance-related genes(19 up-regulated and 8 down-regulated)and 11 photosyn-thetic-related genes(9 u
作者
杨亚杰
李昱樱
申状状
陈天
荣二花
吴玉香
YANG Ya-Jie;LI Yu-Ying;SHEN Zhuang-Zhuang;CHEN Tian;RONG Er-Hua;WU Yu-Xiang(College of Agronomy,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China)
出处
《作物学报》
CAS
CSCD
北大核心
2022年第11期2733-2748,共16页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31171599)
山西省基础研究计划项目(自由探索类)(20210302124154)资助。
关键词
草棉
同源多倍体
流式细胞
生理指标
转录组测序
差异表达基因(DEGs)
Gossypium herbaceum
autopolyploids
flow cytometry
physiological indexs
transcriptome sequencing
differen-tially expressed genes
