摘要
为探讨小麦白粉病的防治新途径，采用不同浓度的牛蒡寡糖（BOS）（0．5～20．0g／L）溶液处理小麦幼苗，分析其感染白粉病菌后病情指数、苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）和过氧化氢酶（CAT）活性的变化。结果表明，随着BOS溶液浓度的升高，小麦幼苗的病情指数越来越低，对白粉病的抗性越来越强，在BOS浓度达到10．0g／L时，抗性最好。BOS处理后的小麦幼苗叶片PAL、POD和CAT活性均高于对照，且在一定浓度范围内酶活性随着BOS浓度的增加而升高。PAL的活性在BOS处理浓度为5．0g／L时达到最大，而POD和CAT活性在10．0g／L时出现峰值。说明适宜浓度的牛蒡寡糖诱导可以提高小麦幼苗内部相关防御酶的活性，进而增强其白粉病抗性。
Powdery mildew is one of the most important disease on wheat. Different concentrations of Burdock Oligosaccharide （BOS） （0.5, 2.0, 5.0, 10. 0 and 20.0 g/L） were sprayed to wheat seedlings at oneleaf stage, water was sprayed as control, the wheat seedlings were inoculated with powdery mildew at three-leaf stage. Seriousness of powdery mildew in different treatments were scored on classes of 0 - 4 when the control were heavily infected, illness index find %） of wheat seedlings were calculated. Activities of phenylalanin ammo-nialyase（PAL）,Peroxidase（POD） and Catalase （CAT） in wheat with different treatments were investigated. According to the the difference of illness index and the activity changes of enzymes, powdery mildew resistance in wheat induced by BOS was analysed. Results showed that powdery mildew resistance in wheat gradually improved along with the increase of concentration of BOS. The resistance were the best when the BOS concentration was 10 g/L. Activities of PAL, POD and CAT were higher than that of control and gradually increased along with the increase of concentration of BOS. Activitie of PAL was the highest when the BOS concentration was 5.0 g/L. When BOS concentration was higher than 5.0 g/L, the activity of PAL decreased. Activities of POD and CAT achieved their peaks when the BOS concentration was 10.0 g/L. It was concluded that BOS induction at appropriate concentration can improve the activites of defensive enzymes and evidently enhance the powdery mildew resistance in wheat.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2009年第3期540-542,共3页
Journal of Triticeae Crops
基金
河南工业大学高层次人才科研基金项目(150318)
关键词
小麦
白粉病菌
牛蒡寡糖
诱导抗性
防御酶
Wheat
Powdery mildew
Burdock oligosaccharide
Induced resistance
Defensive enzyme
