摘要
[目的]对茶叶蛋白质的最佳提取条件进行研究,并研究茶叶蛋白酶解产物的抗氧化性,为茶叶的高附加值利用提供思路。[方法]采用超声波辅助碱法提取茶叶蛋白质,采用响应面法进行茶叶蛋白提取工艺的优化。将茶叶蛋白采用风味蛋白酶进行水解,获得具有抗氧化活性的茶叶多肽。[结果]通过响应面法得到茶叶蛋白的最佳理论提取条件:提取时间120 min,提取温度59.02℃,碱液浓度0.65 mol/L,料液比1∶20。在此条件下,预测得到的茶叶蛋白质最大提取量为29.79%,经过验证后,茶叶蛋白的最大提取量达到28.39%。利用风味蛋白酶酶解茶叶蛋白获得具有较强抗氧化能力的多肽混合物,酶解的最佳条件为:酶解时间60 min,酶解温度60℃,酶解pH 7.0,酶用量9%。[结论]该研究开发了一种茶叶蛋白的提取工艺,并且茶叶蛋白可以通过风味蛋白酶酶解制备绿色、天然的抗氧化剂。
[Objective]To study the optimum extraction conditions of protein from tea and investigate the antioxidant property of the enzymatic hydrolysates of tea protein,so as to provide a new perspective for the high added-value development of tea protein.[Method]The ultrasound-assisted alkali method was used for the extraction of tea protein,and the extraction process was also optimized through the response surface methodology.The antioxidant tea peptides were obtained by hydrolyzing the purified tea protein with flavor protease.[Result]The predicated optimum conditions for the extraction of tea protein were as follows:extraction time of 120 min,extraction temperature of 59.02℃,alkali concentration of 0.65 mol/L,and material/solvent ratio of 1∶20.The predicted maximum protein extracted yield was 29.79%under these conditions.The actual yield of extracted tea protein was 28.39%after verification by experiment.The peptides which were obtained by hydrolyzing the tea protein using flavourzyme demonstrated good antioxidant property.The optimum enzymatic hydrolysis conditions getting from single factor experiment were:the reaction time of 60 min,the reaction temperature of 60℃,the pH value of 7,and the dosage of flavourzyme of 9%.[Conclusion]We successfully developed a new extraction method for the tea protein,and studied the antioxidant property of the enzymatic hydrolysates of tea protein.The results showed that it could be developed to a kind of green natural antioxidant.
作者
张子迪
李宜慧
朱彩霞
杨薇
白东清
李涛
ZHANG Zi-di;LI Yi-hui;ZHU Cai-xia(College of Basic Science,Tianjin Agricultural University,Tianjin 300384)
出处
《安徽农业科学》
CAS
2020年第7期202-206,212,共6页
Journal of Anhui Agricultural Sciences
基金
天津市高等学校大学生创新创业训练计划项目(200810061098)。
关键词
茶
蛋白提取
抗氧化性
响应面优化
Tea
Protein extraction
Antioxidant
Response surface methodology
