摘要
采用碱提酸沉法研究了制取花生分离蛋白的优化条件。结果显示 ,当花生粕的匀浆料液比为 1∶8(m/V)、碱浸提液pH为 8 2、浸提温度为 6 0℃ ,重复浸提两次 ,每次浸提 2h ,酸沉pH为4 5时 ,制取的花生分离蛋白纯度可达 90 2 1% ,蛋白质回收利用率可达 75 74 %。研究结果可为花生蛋白的合理有效利用奠定基础。
Alkaline extraction and acid precipitation were employed to produce peanut protein isolate.The results of single-factor and orthogonal experiments showed the optimum conditions of isolation.After defatted peanut flour was mixed with distilled water in the ratio of 1∶8(m/V) and the pH was adjusted to 8.2,the suspension was extracted at 60℃ for 2 h.Repeating the process above,peanut protein was precipitated to its maximum in the condition of pH4.5.The purity of the isolated protein can reach 90.21% and the utilization ratio is 75.74%.
出处
《中国油脂》
CAS
CSCD
北大核心
2004年第11期21-23,共3页
China Oils and Fats
