摘要
制备了一种分子印迹荧光传感器用于菊酯类农药代谢物间苯氧基苯甲醛(3-PBD)的选择性测定。以3-PBD为模板分子,3-氨丙基三乙氧基硅烷为功能单体,四乙氧基硅烷为交联剂,采用溶胶-凝胶法在Mn掺杂ZnS量子点表面成功合成了荧光分子印迹聚合物。采用红外光谱、X射线衍射及X射线光电子能谱对聚合物进行表征,并对检测条件进行了优化。在最佳条件下,该荧光传感器对3-PBD的响应线性范围为0.3~5μmol/L,检出限为0.267μmol/L。此分子印迹荧光传感器可与农药的酶解过程相结合,用于菊酯类农药的快速检测。对实际样品的测定结果显示,3-PBD的回收率为84.0%~119%,相对标准偏差不超过5.0%。结果表明,该分子印迹荧光传感器可以用于食品中3-PBD的测定。
A molecularly imprinted fluorescence sensor was prepared for the selective determination of the pyrethroid pesticide metabolite 3-phenoxybenzaldehyde.Fluorescent molecularly imprinted polymers were successfully synthesized by sol-gel method on the surface of Mn-doped ZnS quantum dots,with 3-phenoxybenzaldehyde as template molecule,3-aminopropyl triethoxysilane and tetraethyl orthosilicate as functional monomer and cross linker,respectively.The polymers were characterized by infrared spectroscopy,X-ray diffraction and X-ray photoelectron spectroscopy,and the detection conditions were optimized.Results showed that under the optimal conditions,the linear response range for the fluorescence sensor to 3-phenoxybenzaldehyde ranged from 0.3 μmol/L to 5 μmol/L,with a detection limit of 0.267 μmol/L.Combined with the enzymatic hydrolysis process of pesticides,this molecularly imprinted fluorescence sensor could be used in the rapid detection of pyrethroidpesticides.Results indicated that recoveries for 3-phenoxybenzaldehyde were in the range of 84.0%-119%,with the relative standard deviations not more than 5.0%.The prepared molecularly imprinted fluorescence sensor could be used for the determination of 3-phenoxybenzaldehyde in food.
作者
季芯羽
叶泰
袁敏
曹慧
徐斐
于劲松
任华
叶寅颖
李嘉怡
王彦茜
JI Xin-yu;YE Tai;YUAN Min;CAO Hui;XU Fei;YU Jin-song;REN Hua;YE Yin-ying;LI Jia-yi;WANG Yan-qian(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2019年第12期1487-1492,共6页
Journal of Instrumental Analysis
基金
国家重点研发计划(2017YFC1600603)
上海市科委科技支撑(农业重点科技攻关)项目(17391901500)
上海大学生创新创业训练计划项目(SH2018142).
