摘要
采用生物矿化策略,在磷酸盐缓冲液中制备了猪肝酯酶(Porcine liver esterase,PLE)杂化"纳米花"(Ca3(PO4)2-PLE)。利用扫描电镜、元素能谱分析及红外光谱对所制备的杂化"纳米花"进行了形貌和成分分析。对杂化"纳米花"制备过程中的参数进行优化,结果表明:在10 mmol/L磷酸盐缓冲液(p H 7. 4)中,猪肝酯酶质量浓度为0. 3 g/L,钙离子浓度为500 mmol/L,制备温度为20℃时,所制备的Ca3(PO4)2-PLE具有最高酶活,其酶活为同等酶浓度下液酶酶活的169%。将制备的Ca3(PO4)2-PLE用于4种菊酯类农药的酶解性能研究,发现相同条件下,Ca3(PO4)2-PLE的酶解率均优于液酶。此外,制备的Ca3(PO4)2-PLE用于菊酯农药酶解循环2次后仍保持70%以上的活力。
In this review,the porcine liver esterase(PLE)organic-inorganic hybrid nanoflowers(Ca 3(PO 4)2-PLE)were prepared in phosphate buffer by biomineralization strategy.The morphology and components of the prepared organic-inorganic hybrid nanoflowers were analyzed by scanning electron microscopy(SEM),energy dispersive spectrometry(EDS)and Fourier transform infrared spectroscopy(FT-IR).The parameters for the preparation of PLE hybrid nanoflowers were optimized.Results showed that the prepared PLE hybrid nanoflowers had the highest enzyme activity in phosphate buffer solution(pH 7.4)of 10 mmol/L,PLE concentration of 0.3 g/L and calcium ion concentration of 500 mmol/L at preparation temperature of 20℃,and its enzyme activity was 169%of the free enzyme in the same enzyme concentration.Furthermore,the PLE hybrid nanoflowers were used to study the enzymatic hydrolysis properties toward four pyrethroid pesticides.It was found that under the same conditions,the enzymatic hydrolysis rates of PLE hybrid nanoflowers were better than that of the free enzyme.In addition,the prepared PLE hybrid nanoflowers still remained over 70%of the enzymatic activity after twice uses of the pyrethroid pesticide enzymatic cycle.
作者
彭开敏
叶泰
曹慧
袁敏
于劲松
徐斐
PENG Kai-min;YE Tai;CAO Hui;YUAN Min;YU Jin-song;XU Fei(Shanghai Engineering Research Center of Food Microbiology,School of Medical Instruments and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2018年第12期1412-1417,共6页
Journal of Instrumental Analysis
基金
“十三五”国家重点研发计划(2017YFC1600603)
上海市科委科技支撑(农业重点科技攻关)项目(17391901500).
关键词
杂化纳米花
猪肝酯酶(PLE)
菊酯类农药
酶活力
酶解
hybrid nanoflowers
porcine liver esterase(PLE)
pyrethroid pesticide
enzyme activity
enzymolysis
