摘要
以6-羟基烟酸(6-HNA)为模板,采用分子印迹溶胶-凝胶法合成了具有良好光学性质的印迹Mn掺杂Zn S量子点磷光传感器(MIP-QDs)。红外光谱(FT-IR)和扫描电镜(SEM)测试表明,印迹聚合物成功接枝在纳米传感器表面。吸附试验显示,MIP-QDs对6-HNA的亲和性与选择性显著高于其结构类似物阔马酸和烟酸,具备作为检测探针的潜力。在最优条件下,基于6-HNA可选择性猝灭MIP-QDs磷光而建立了灵敏、简便的检测微量6-HNA的光学新方法。当6-HNA浓度在3.4~67.0μmol/L范围内,MIP-QDs的磷光猝灭率P_0/P随浓度变化的关系符合Stern-Volmer方程(r^2=0.996 5),检出限为1.03μmol/L。实际样品测定显示,6-HNA的回收率为91.0%~103.9%,相对标准偏差不超过4.5%,检测结果与HPLC法相一致(P>0.05),方法具有良好的准确性和可行性。
An optical nanosensor based Mn doped ZnS quantum dots（QDs）was synthesized using 6-hydroxynictinic acid（6-HNA） as template via surface molecular imprinting sol-gel method.Fourier transform infrared spectroscopic（FT-IR） spectra and scanning electron microscopic（SEM） images showed that the imprinted polymer was successfully grafted onto MIP-QDs.Adsorption tests revealed that the affinity and selectivity of MIP-QDs for 6-HNA were significantly higher than its analogues nicotinic acid and coumalic acid.Under the optimized conditions,a phosphorescent sensor for detecting 6-HNA was constructed.The linear range for detection of 6-HNA was in the range of 3.4-67.0 μmol/L with a detection limit of 1.03 μmol/L and a correlation coefficient（r2） of 0.996 5.Recoveries of 91.0%-103.9% and relative standard deviations less than 4.5% were achieved in real samples,which were in good agreement with that of the high performance liquid chromatographic（HPLC） method（P〉0.05）.The proposed method had good veracity and feasibility.
出处
《分析测试学报》
CAS
CSCD
北大核心
2017年第4期478-483,共6页
Journal of Instrumental Analysis
基金
国家自然科学基金项目(31371813)
国家科技部(863计划)项目资助(2013FY113400)
关键词
量子点
分子印迹
磷光检测
6-羟基烟酸(6-HNA)
烟酸
quantum dots
molecular imprinting
phosphorescence detection
6-hydroxynicotinicacid （6-HNA）
nicotinic acid
