摘要
目的探讨高尔基糖蛋白-73(GP73)和血管内皮生长因子(VEGF)在原发性肝癌(PHC)和正常肝脏组织中的表达及对PHC的诊断价值。方法标本来源:PHC患者36例、肝硬化患者30例和肝血管瘤患者30例作为对照,采用免疫组化法检测以上标本GP73及VEGF表达水平;同时采用酶联免疫吸附试验(ELISA)检测血清GP73及VEGF水平。进一步比较PHC患者肿瘤大小、高危转移、低危转移等病理特征下血清GP73及VEGF水平的差异。结果PHC肝组织中GP73及VEGF表达显著高于肝硬化肝组织及正常肝组织及癌旁组织。36例PHC肝组织GP73表达阳性的有28例,而肝硬化组织、正常肝组织GP73表达阳性分别为8例和2例,PHC肝组织GP73表达量为185.52±29.14μg/L,而肝硬化组织、正常肝组织GP73表达量分别为59.73±16.26μg/L和43.90±10.17μg/L,χ^2=29.16,p=0.027;36例PHC肝组织VEGF表达阳性的有22例,而肝硬化组织、正常肝组织VEGF表达阳性分别为9例和3例,PHC肝组织VEGF表达量为493.41±75.60ng/L,而肝硬化组织、正常肝组织VEGF表达量分别为197.09±38.28 ng/L和168.76±29.75 ng/L,χ^2=12.38,p=0.019。高危转移组患者GP73及VEGF表达明显高于低危转移组。高危转移组患者GP73、VEGF表达量分别为274.05±45.43μg/L和706.08±115.39 ng/L,而低危转移组患者GP73、VEGF表达量分别为150.98±23.50μg/L和527.41±91.60ng/L。肿瘤直径大于5cm的GP73及VEGF的表达明显高于肿瘤直径小于5cm的表达。肿瘤直径大于5cm的GP73、VEGF表达量分别为201.83±36.27μg/L和484.19±72.28ng/L,肿瘤直径小于5cm的GP73、VEGF表达量分别为173.52±27.54μg/L和418.31±69.12ng/L。且GP73的表达与VEGF有正相关关系(p=0.013,0.022,r=0.87)。结论GP73可用于HPC高危人群的普查和筛选,是单独检测HPC的理想血清标志物,VEGF可促进肿瘤的增生和转移;在PHC的生长过程中,随着GP73表达量由低到高,VEGF促进肿瘤细胞生长的作用增强。因此,联合GP73、VEGF检测对于HPC的�
Objective To detect the expression of Golgi protein 73(GP73)and vascular endothelial growth factor(VEGF)in the primary hepatic cancer(PHC)tissue and normal liver tissue,so as to explore the significance of their dignosis value to HPC.Methods The source of the specimens:36 cases of patients with PHC,30 cases of patients with hepatic cirrhosis and 30 cases of patients with liver hemangioma as control.Then GP73 and VEGF of specimens above the were expressed by Immunohistochemical staining,At the same time,levels of GP73 and VEGF in the serum were detected by Enzyme-linked immunosorbent assay.Further the difference between the patients with tumor size,metastasis,PHC risk low risk transfer under pathological characteristics of serum GP73 and VEGF levelswere compared.Results Liver tissue PHC expression of GP73 and VEGF were significantly higher than that of liver cirrhosis and normal liver tissues and para carcinoma tissues.GP73 expression in 28 cases of liver tissue is positive In 36 cases,while GP73 positive expression in liver cirrhosis tissues and normal liver tissue were 8 cases and 2 cases respectively.Expression levels of GP73 in liver tissue PHC was 185.52±29.14 g/L.However,GP73 expression in cirrhosis tissues and normal liver tissue were 59.73±16.26μg/L and 43.90±10.17 ng/L respectively,x^2=29.16,p=0.027.22 cases of VEGF expression is positive in 36 cases in PHC liver tissue,while in liver cirrhosis tissues and normal liver tissue were 9 cases and 3 cases respectively.VEGF expression in liver tissue of PHC was 493.41±75.60ng/L,while 197.09±38.28 ng/L and 168.76±29.75 ng/L respectively in cirrhosis tissues and normal liver tissue,x^2=12.38,p=0.019.GP73 and VEGF expression was significantly higher in high risk transfer group of patients than the low risk transfer group.GP73 and VEGF expressions in liver tissue of high risk transfer PHC were 274.05±45.43μg/L and 706.08+115.39μg/L respectively,while150.98±23.50ng/L and 527.41±91.60ng/Lng/L respectively in liver tissue of low risk transfer PHC;Expressio
作者
吴雨梅
黄震
李娜
方宏才
WU Yumei;HUANG Zhen;LI Na;FANG Hongcai
出处
《九江学院学报（自然科学版）》
CAS
2019年第4期88-92,共5页
Journal of Jiujiang University：Natural Science Edition
