摘要
目的:比较不同类型凋亡基因在牙龈瘤中的特征性表达,探讨细胞凋亡在牙龈瘤发病中的作用机制。方法:2017年12月-2018年5月治疗36例牙龈瘤患者,收集对应的牙龈瘤组织和正常牙龈组织。其中6对用PCR芯片检测84个凋亡相关基因的表达,30对用实时荧光定量PCR验证芯片结果。根据内参基因ΔΔCt法计算基因表达改变,采用SPSS 17.0软件包对数据进行统计学分析。结果:与正常牙龈组织相比,牙龈瘤组织中AIFM1、BCL2、BCL2L1、BCL2L2、BFAR、BIRC2、BIRC3、BIRC6、BNIP2、BNIP3、CD40LG、XIAP表达升高,TNFRSF25表达下降。结论:BCL-2家族抗凋亡基因和IAP家族基因过表达,抑制牙龈组织正常凋亡的发生,最终引起牙龈瘤。
PURPOSE:To investigate the characteristic expression of apoptotic genes in epulis.METHODS:Thirty-six patients with epulis were recruited in this study,and related tumor and normal gingival tissues were collected.Six pairs of samples were used for PCR array analysis of apoptosis,and the other thirty pairs of samples were used for qRT-PCR replication.The expression levels of related genes were calculated with internal reference gene delta ΔΔCt.The data were analyzed with SPSS 17.0 software package.RESULTS:Compared with the normal gingival tissues,the expression levels of AIFM1,BCL2,BCL2 L1,BCL2 L2,BFAR,BIRC2,BIRC3,BIRC6,BNIP2,BNIP3,CD40 LG,and XIAP were significantly increased in the gingival tissues,while the expression level of TNFRSF25 was decreased.CONCLUSIONS:Over-expression of anti-apoptotic genes in Bcl-2 family and IAP family inhibits apoptosis of gingival tissues,which eventually causes epulis.
作者
蒋泱泱
徐斌
房兵
JIANG Yang-yang;XU Bin;FANG Bing(Department of Orthodontics,Shanghai Ninth People s Hospital,College of Stomatology,Shanghai Jiao Tong University School of Medicine,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology&Shanghai Research Institute of Stomatology,Shanghai 200011;Department of Stomatology,Ningbo Second People's Hospital,Ningbo 315010,Zhejiang Province,China)
出处
《上海口腔医学》
CAS
CSCD
北大核心
2020年第1期31-35,共5页
Shanghai Journal of Stomatology
基金
浙江省病理生理学技术研究重点实验室开放课题（201814）
